Real-time reverse transcriptase polymerase chain reaction assays for Middle East Respiratory Syndrome.

Autor: Douglas CE; Diagnostic Systems Division, United States Army Medical Research Institute of Infectious Disease, Fort Detrick, Maryland, USA., Kulesh DA; Diagnostic Systems Division, United States Army Medical Research Institute of Infectious Disease, Fort Detrick, Maryland, USA., Jaissle JG; Diagnostic Systems Division, United States Army Medical Research Institute of Infectious Disease, Fort Detrick, Maryland, USA., Minogue TD; Diagnostic Systems Division, United States Army Medical Research Institute of Infectious Disease, Fort Detrick, Maryland, USA. Electronic address: timothy.d.minogue.civ@mail.mil.
Jazyk: angličtina
Zdroj: Molecular and cellular probes [Mol Cell Probes] 2015 Dec; Vol. 29 (6), pp. 511-513. Date of Electronic Publication: 2015 Sep 11.
DOI: 10.1016/j.mcp.2015.09.001
Abstrakt: Here we designed and tested two highly specific quantitative TaqMan(®)-MGB-based reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) assays for Middle East Respiratory Syndrome (MERS). The primers and probes for these assays were evaluated and found to have a limit of detection (LOD) of 0.005 plaque-forming units/PCR (pfu/PCR).
(Published by Elsevier Ltd.)
Databáze: MEDLINE
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