| Autor: |
Naglot A; Defence Research Laboratory, Post Bag No. 2, Tezpur (Assam), India., Goswami S; Defence Research Laboratory, Post Bag No. 2, Tezpur (Assam), India., Rahman I; Defence Research Laboratory, Post Bag No. 2, Tezpur (Assam), India., Shrimali DD; Defence Research Laboratory, Post Bag No. 2, Tezpur (Assam), India., Yadav KK; Defence Research Laboratory, Post Bag No. 2, Tezpur (Assam), India., Gupta VK; Defence Research Laboratory, Post Bag No. 2, Tezpur (Assam), India., Rabha AJ; Defence Research Laboratory, Post Bag No. 2, Tezpur (Assam), India., Gogoi HK; Defence Research Laboratory, Post Bag No. 2, Tezpur (Assam), India., Veer V; Defence Research Laboratory, Post Bag No. 2, Tezpur (Assam), India. |
| Abstrakt: |
Indigenous strains of Trichoderma species isolated from rhizosphere soils of Tea gardens of Assam, north eastern state of India were assessed for in vitro antagonism against two important tea fungal pathogens namely Pestalotia theae and Fusarium solani. A potent antagonist against both tea pathogenic fungi, designated as SDRLIN1, was selected and identified as Trichoderma viride. The strain also showed substantial antifungal activity against five standard phytopathogenic fungi. Culture filtrate collected from stationary growth phase of the antagonist demonstrated a significantly higher degree of inhibitory activity against all the test fungi, demonstrating the presence of an optimal blend of extracellular antifungal metabolites. Moreover, quantitative enzyme assay of exponential and stationary culture filtrates revealed that the activity of cellulase, β-1,3-glucanase, pectinase, and amylase was highest in the exponential phase, whereas the activity of proteases and chitinase was noted highest in the stationary phase. Morphological changes such as hyphal swelling and distortion were also observed in the fungal pathogen grown on potato dextrose agar containing stationary phase culture filtrate. Moreover, the antifungal activity of the filtrate was significantly reduced but not entirely after heat or proteinase K treatment, demonstrating substantial role of certain unknown thermostable antifungal compound(s) in the inhibitory activity. |
