In vitro effects of extracellular hypercapnic acidification on the reactivity of rat aorta: Rat aorta vasodilation during hypercapnic acidification.

Autor: de Nadai TR; Department of Surgery and Anatomy, Ribeirão Preto School of Medicine, University of São Paulo (FMRP-USP), Ribeirão Preto, SP, Brazil., de Nadai MN; Department of Surgery and Anatomy, Ribeirão Preto School of Medicine, University of São Paulo (FMRP-USP), Ribeirão Preto, SP, Brazil., Cassiano Silveira AP; Department of Surgery and Anatomy, Ribeirão Preto School of Medicine, University of São Paulo (FMRP-USP), Ribeirão Preto, SP, Brazil., Celotto AC; Department of Surgery and Anatomy, Ribeirão Preto School of Medicine, University of São Paulo (FMRP-USP), Ribeirão Preto, SP, Brazil., Albuquerque AA; Department of Surgery and Anatomy, Ribeirão Preto School of Medicine, University of São Paulo (FMRP-USP), Ribeirão Preto, SP, Brazil., de Carvalho MT; Department of Surgery and Anatomy, Ribeirão Preto School of Medicine, University of São Paulo (FMRP-USP), Ribeirão Preto, SP, Brazil., Scarpelini S; Department of Surgery and Anatomy, Ribeirão Preto School of Medicine, University of São Paulo (FMRP-USP), Ribeirão Preto, SP, Brazil., Rodrigues AJ; Department of Surgery and Anatomy, Ribeirão Preto School of Medicine, University of São Paulo (FMRP-USP), Ribeirão Preto, SP, Brazil., Evora PR; Department of Surgery and Anatomy, Ribeirão Preto School of Medicine, University of São Paulo (FMRP-USP), Ribeirão Preto, SP, Brazil. Electronic address: prbevora@gmail.com.
Jazyk: angličtina
Zdroj: Nitric oxide : biology and chemistry [Nitric Oxide] 2015 Nov 15; Vol. 50, pp. 79-87. Date of Electronic Publication: 2015 Sep 07.
DOI: 10.1016/j.niox.2015.09.001
Abstrakt: The mechanisms by which pH influences vascular tone are not entirely understood, but evidence suggests that the endothelium is involved. Here, we aimed to study the in vitro vascular responses induced by extracellular hypercapnic acidification (HA), as well as the endothelium-dependent mechanisms that are involved in the responses. We bubbled a mixture of CO 2 (40%)/O 2 (60%) in an organ bath; we constructed a pH-response curve (pH range 7.4-6.6) and registered isometric force simultaneously. Aortic rings from rats were pre-contracted with phenylephrine (10 -6  M) and incubated for 30 min in the presence of different chemicals. The relaxations induced by HA occurred in rings with endothelium were: 1) Partially inhibited by indomethacin (10 -5  M) (PGI 2 pathway inhibitor); 2) Strongly inhibited by NO pathways: L-NAME (10 -4  M) and L-NMMA (10 -4  M) (no specific NO synthase inhibitors); L-Nil (10 -3  M) (specific iNOS inhibitor); ODQ (10 -4  M) (specific guanylate cyclase inhibitor), and; 4) Inhibit by tetraethylammonium (10 -3  M) (non-specific potassium channel inhibitor), glibenclamide (10 -5  M) (specific K AT P inhibitor), aminopyridine (10 -3  M) (specific Kv inhibitor) and apamin (10 -6  M) (specific SKCa inhibitor).
In Conclusion: 1) HA causes endothelium-dependent relaxation; 2) Indomethacin failed in blocking this relaxation, but the method limitation does not allow ruling out some prostanoid role; 3) The HA vessel relaxation is mediated via cGMP/NO, and; 4) The hyperpolarization occurs by the action of potassium SK Ca , K ATP and K v channels without relying on BK Ca channels.
(Copyright © 2015 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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