(1→6)- and (1→3)(1→6)-β-glucans from Lasiodiplodia theobromae MMBJ: Structural characterization and pro-inflammatory activity.

Autor: Oliveira KS; Departamento de Ciências Biológicas, Universidade Estadual Paulista - UNESP (FCL-Assis), CEP 19806-900 Assis, SP, Brazil., Di Bastiani M; Departamento de Ciências Biológicas, Universidade Estadual Paulista - UNESP (FCL-Assis), CEP 19806-900 Assis, SP, Brazil., Cordeiro LM; Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, CP 19.046, CEP 81.531-980 Curitiba, PR, Brazil. Electronic address: lucimaramcc@ufpr.br., Costa MF; Departamento de Ciências Biológicas, Universidade Estadual Paulista - UNESP (FCL-Assis), CEP 19806-900 Assis, SP, Brazil., Toledo KA; Departamento de Ciências Biológicas, Universidade Estadual Paulista - UNESP (FCL-Assis), CEP 19806-900 Assis, SP, Brazil., Iacomini M; Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, CP 19.046, CEP 81.531-980 Curitiba, PR, Brazil., Babosa AM; Departamento de Química, Universidade Estadual de Londrina, CEP 86051-990 Londrina, PR, Brazil., Dekker RF; Biorefining and Biotechnology Consultancy, Rua João Huss 200, Gleba Palhano, CEP 86050-490 Londrina, PR, Brazil., Nascimento VM; Departamento de Ciências Biológicas, Universidade Estadual Paulista - UNESP (FCL-Assis), CEP 19806-900 Assis, SP, Brazil.
Jazyk: angličtina
Zdroj: Carbohydrate polymers [Carbohydr Polym] 2015 Nov 20; Vol. 133, pp. 539-46. Date of Electronic Publication: 2015 Jul 26.
DOI: 10.1016/j.carbpol.2015.07.060
Abstrakt: The chemical composition and structural characterization of exopolysaccharides from the fungus Lasiodiplodia theobromae MMBJ are described, and the immunomodulatory activity of a purified β-glucan was evaluated. L. theobromae MMBJ produced three different β-glucans. One, fraction PEPS, was a branched (1→3)(1→6)-β-glucan and was insoluble in cold water. The other two, fractions SEPS-005R and SEPS-10E, were characterized as linear (1→6)-β-glucans with molar mass of 1.8×10(6)Da and 7.0×10(3)Da, respectively. From a total of 2.2g/L of EPS produced by L. theobromae through submerged fermentation, 1.5g/L (67%) was of the branched (1→3)(1→6)-β-glucan, while 25% (w/w) were linear (1→6)-β-glucans. Tests conducted with macrophages showed that the high molar mass (1→6)-β-glucan fraction (SEPS-005R) induced a pro-inflammatory response pattern.
(Copyright © 2015 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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