The effect of surfactant on fermentation of kitasamycin in Streptomyces kitasatoensis.

Autor: Zheng Q; State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, People's Republic of China., Gao S; State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, People's Republic of China.
Jazyk: angličtina
Zdroj: Biotechnology and applied biochemistry [Biotechnol Appl Biochem] 2016 Nov; Vol. 63 (6), pp. 895-900. Date of Electronic Publication: 2015 Nov 17.
DOI: 10.1002/bab.1443
Abstrakt: Soybean oil is an important carbon source in kitasamycin fermentation by Streptomyces kitasatoensis. In this study, three different surfactants, Tween 80, Tween 85, and sodium dodecyl sulfate (SDS), were added in the fermentation medium to improve soybean oil utilization. Results indicated that all of these surfactants promote kitasamycin biosynthesis. When 0.5 g/L SDS was added at the beginning of fermentation, kitasamycin production increased by 55% and A 5 content improved by 12%, compared with the control treatment (i.e., no surfactant added). Oil consumption rate and lipase activity were also improved in the presence of SDS, producing more organic acids benefiting kitasamycin biosynthesis. High butyric acid concentration in the fermentation medium containing SDS repressed C-3 acetylation and promoted A 5 component accumulation. Additionally, utilization of oil components by S. kitasatoensis was altered. Specifically, linoleic acid was primarily used in the fermentation process with SDS, whereas oleic acid was primarily used in the fermentation process where no surfactant had been added.
(© 2015 International Union of Biochemistry and Molecular Biology, Inc.)
Databáze: MEDLINE
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