| Autor: |
Rabiatul AR; a Tissue Engineering Centre, Universiti Kebangsaan Malaysia Medical Centre , Universiti Kebangsaan Malaysia , Kuala Lumpur , Malaysia., Lokanathan Y; a Tissue Engineering Centre, Universiti Kebangsaan Malaysia Medical Centre , Universiti Kebangsaan Malaysia , Kuala Lumpur , Malaysia., Rohaina CM; a Tissue Engineering Centre, Universiti Kebangsaan Malaysia Medical Centre , Universiti Kebangsaan Malaysia , Kuala Lumpur , Malaysia., Chowdhury SR; a Tissue Engineering Centre, Universiti Kebangsaan Malaysia Medical Centre , Universiti Kebangsaan Malaysia , Kuala Lumpur , Malaysia., Aminuddin BS; b Ear, Nose and Throat Consultant Clinic, Ampang Puteri Specialist Hospital , Ampang , Selangor , Malaysia., Ruszymah BH; a Tissue Engineering Centre, Universiti Kebangsaan Malaysia Medical Centre , Universiti Kebangsaan Malaysia , Kuala Lumpur , Malaysia.; c Faculty of Medicine, Department of Physiology , Universiti Kebangsaan Malaysia , Kuala Lumpur , Malaysia. |
| Abstrakt: |
Scaffold design is an important aspect of in vitro model development. In this study, nanoscaffold surface modification, namely UV radiation and genipin cross-linking to immobilize collagen on the surface of electrospun poly (methyl methacrylate) (PMMA) nanofiber sheet was investigated. Samples were divided into four groups; PMMA nanofibers (PMMA), collagen-coated PMMA nanofibers (PMMACOL), genipin cross-linked collagen-coated PMMA nanofibers (PMMAGEN), and UV-irradiated collagen-coated PMMA nanofibers (PMMAUV). 6 h of UV radiation significantly reduced the hydrophobicity of PMMA nanofibers from (131.88° ± 1.33°) to (110.04° ± 0.27°) (p < 0.05). The amount of collagen immobilized was significantly higher in PMMAGEN group (239.36 ± 16.63 μg collagen/mg nanofibers) (p < 0.05) compared to the other groups. RECs on all scaffold expressed epithelial cell-specific markers (CK18 and CK14), mucin-producing cell marker (MUC5Ac) and were actively proliferating, based on the positive expression of Ki67. Total number of attached cells was significantly the highest in PMMAUV group on day 9 (6.44 × 10(4) ± 2.77 × 10(4) cells/cm(2)) and it has the highest proliferation rate from day 4 to 9 (0.005 ± 0.003 h(-1)) compared to the other groups. Even though PMMAGEN group showed the highest collagen adsorption, in terms of cells attachment and proliferation, PMMAUV group showed a better outcome compared to the other groups. Thus, PMMAUV scaffold is more suitable to be used in the construction of in vitro respiratory epithelial model. |
