Dried Blood Spots: A Tool to Ensure Broad Access to Hepatitis C Screening, Diagnosis, and Treatment Monitoring.

Autor:	Soulier A; National Reference Center for Viral Hepatitis B, C, and Delta, Department of Virology INSERM U955, Centre Hospitalier Intercommunal de Créteil, France., Poiteau L; National Reference Center for Viral Hepatitis B, C, and Delta, Department of Virology INSERM U955, Centre Hospitalier Intercommunal de Créteil, France., Rosa I; Department of Hepatology and Gastroenterology, Centre Hospitalier Intercommunal de Créteil, France., Hézode C; Department of Hepatology and Gastroenterology, Hôpital Henri Mondor, Université Paris-Est INSERM U955, Centre Hospitalier Intercommunal de Créteil, France., Roudot-Thoraval F; Department of Public Health, Hôpital Henri Mondor, Université Paris-Est INSERM U955, Centre Hospitalier Intercommunal de Créteil, France., Pawlotsky JM; National Reference Center for Viral Hepatitis B, C, and Delta, Department of Virology INSERM U955, Centre Hospitalier Intercommunal de Créteil, France., Chevaliez S; National Reference Center for Viral Hepatitis B, C, and Delta, Department of Virology INSERM U955, Centre Hospitalier Intercommunal de Créteil, France.
Jazyk:	angličtina
Zdroj:	The Journal of infectious diseases [J Infect Dis] 2016 Apr 01; Vol. 213 (7), pp. 1087-95. Date of Electronic Publication: 2015 Sep 02.
DOI:	10.1093/infdis/jiv423
Abstrakt:	Background: With the advent of highly efficient antiviral therapies for hepatitis C virus (HCV) infection, providing broad access to diagnosis and care is needed. The dried blood spot (DBS) technique can be used to collect, store, and ship whole-blood specimens. Our goal was to assess the performance of standardized HCV diagnostic and monitoring tools in the analysis of DBS. Methods: Serum specimens and whole-blood specimens collected using the DBS technique from >500 patients were tested for virological markers used to diagnose and monitor HCV infection. Results: Enzyme immunoassay detection of anti-HCV antibodies in specimens from DBS was reliable after establishment of a new signal-to-cutoff ratio. HCV RNA was detected DBS from the vast majority of patients with active replication, but HCV RNA levels were substantially lower than in serum specimens, implying that only the presence or absence of HCV RNA or changes in the HCV RNA level should be taken into consideration for therapy. Detection of HCV core antigen in specimens from DBS was not a sensitive marker of chronic HCV infection. HCV genotype determination was possible in the vast majority of DBS. Conclusions: This study shows that whole-blood specimens collected using the DBS technique can be confidently used to diagnose and monitor HCV infection. DBS could help improve access to care for HCV infection because they are suitable for use in large-scale screening programs, diagnosis, and therapeutic monitoring. (© The Author 2015. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail journals.permissions@oup.com.)
Databáze:	MEDLINE
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