| Autor: |
Jyotsana B; 1 Animal Biotechnology Section, Central Sheep and Wool Research Institute , Avikanagar-304501 (Rajasthan), India ., Sahare AA; 2 Animal Biotechnology Centre, National Dairy Research Institute , Karnal-132001 (Haryana), India ., Raja AK; 2 Animal Biotechnology Centre, National Dairy Research Institute , Karnal-132001 (Haryana), India ., Singh KP; 2 Animal Biotechnology Centre, National Dairy Research Institute , Karnal-132001 (Haryana), India ., Singla SK; 2 Animal Biotechnology Centre, National Dairy Research Institute , Karnal-132001 (Haryana), India ., Chauhan MS; 2 Animal Biotechnology Centre, National Dairy Research Institute , Karnal-132001 (Haryana), India ., Manik RS; 2 Animal Biotechnology Centre, National Dairy Research Institute , Karnal-132001 (Haryana), India ., Palta P; 2 Animal Biotechnology Centre, National Dairy Research Institute , Karnal-132001 (Haryana), India . |
| Abstrakt: |
We compared the cloning efficiency of buffalo embryos produced by handmade cloning (HMC) using ear skin- and milk-derived donor cells. The blastocyst rate was lower (p < 0.05) for milk-derived than that for skin-derived embryos, whereas the total cell number and apoptotic index were similar. The global level of H3K9ac was higher (p < 0.05) in skin- than in milk-derived cells, whereas the level of H3K27me3 was similar in the two groups. The global level of H3K9ac was similar between milk-derived and in vitro-fertilized (IVF) blastocysts, which was higher (p < 0.05) than that in skin-derived blastocysts. The level of H3K27me3 was similar among the three groups. The expression level of IGF-1R and G6PD was higher (p < 0.05) in skin- than in milk-derived cells, whereas DNMT1, DNMT3a, and HDAC1 expression level was similar. In the blastocysts, the expression level of DNMT1, HDAC1, OCT4, and CDX2 was higher (p < 0.05) in skin-derived than that in IVF blastocysts. The expression level of DNMT3a and IGF-1R, was in the order (p < 0.05) skin-derived and IVF > milk-derived blastocysts and that of NANOG was (p < 0.05) IVF-> milk-derived > skin-derived blastocysts. The expression level of all these genes, except NANOG, was lower (p < 0.05) in milk- than in skin-derived or IVF blastocysts. In conclusion, milk-derived cells can be used for producing HMC embryos of quality similar to that of skin-derived embryos, although with a lower blastocyst rate. |
