The silencing suppressor (NSs) protein of the plant virus Tomato spotted wilt virus enhances heterologous protein expression and baculovirus pathogenicity in cells and lepidopteran insects.

Autor: de Oliveira VC; Department of Cell Biology, University of Brasília, Brasília, Distrito Federal, Brazil. virginiaoliveira@uft.edu.br., da Silva Morgado F; Department of Cell Biology, University of Brasília, Brasília, Distrito Federal, Brazil., Ardisson-Araújo DM; Department of Cell Biology, University of Brasília, Brasília, Distrito Federal, Brazil., Resende RO; Department of Cell Biology, University of Brasília, Brasília, Distrito Federal, Brazil., Ribeiro BM; Department of Cell Biology, University of Brasília, Brasília, Distrito Federal, Brazil.
Jazyk: angličtina
Zdroj: Archives of virology [Arch Virol] 2015 Nov; Vol. 160 (11), pp. 2873-9. Date of Electronic Publication: 2015 Sep 01.
DOI: 10.1007/s00705-015-2580-2
Abstrakt: In this work, we showed that cell death induced by a recombinant (vAcNSs) Autographa californica multiple nucleopolyhedrovirus (AcMNPV) expressing the silencing suppressor (NSs) protein of Tomato spotted wilt virus (TSWV) was enhanced on permissive and semipermissive cell lines. The expression of a heterologous gene (firefly luciferase) during co-infection of insect cells with vAcNSs and a second recombinant baculovirus (vAgppolhfluc) was shown to increase when compared to single vAgppolhfluc infections. Furthermore, the vAcNSs mean time-to-death values were significantly lower than those for wild-type AcMNPV on larvae of Spodoptera frugiperda and Anticarsia gemmatalis. These results showed that the TSWV-NSs protein could efficiently increase heterologous protein expression in insect cells as well as baculovirus pathogenicity and virulence, probably by suppressing the gene-silencing machinery in insects.
Databáze: MEDLINE
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