BCL-2 modulates the unfolded protein response by enhancing splicing of X-box binding protein-1.

Autor: Chonghaile TN; Apoptosis Research Centre, National University of Ireland, Galway, Ireland; School of Natural Sciences, National University of Ireland, Galway, Ireland., Gupta S; Apoptosis Research Centre, National University of Ireland, Galway, Ireland; School of Medicine, National University of Ireland, Galway, Ireland., John M; Apoptosis Research Centre, National University of Ireland, Galway, Ireland; School of Natural Sciences, National University of Ireland, Galway, Ireland., Szegezdi E; Apoptosis Research Centre, National University of Ireland, Galway, Ireland; School of Natural Sciences, National University of Ireland, Galway, Ireland., Logue SE; Apoptosis Research Centre, National University of Ireland, Galway, Ireland; School of Natural Sciences, National University of Ireland, Galway, Ireland. Electronic address: susan.logue@nuigalway.ie., Samali A; Apoptosis Research Centre, National University of Ireland, Galway, Ireland; School of Natural Sciences, National University of Ireland, Galway, Ireland. Electronic address: afshin.samali@nuigalway.ie.
Jazyk: angličtina
Zdroj: Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2015 Oct 09; Vol. 466 (1), pp. 40-5. Date of Electronic Publication: 2015 Aug 28.
DOI: 10.1016/j.bbrc.2015.08.100
Abstrakt: Accumulation of unfolded proteins within the endoplasmic reticulum (ER) triggers a highly conserved stress response mechanism termed the unfolded protein response (UPR). The UPR is a complex series of signaling pathways controlled by ER localized transmembrane receptors, PERK, ATF6 and IRE1α. Following activation IRE1α splices XBP-1 mRNA facilitating the formation of a potent transcription factor, spliced XBP-1. The BCL-2 family members, BAX and BAK, in addition to the mitochondrion also localize to the ER and have been demonstrated to directly interact with IRE1α promoting its activity. In this study we show that in addition to BAX and BAK, the anti-apoptotic BCL-2 protein can regulate IRE1α activity. Enhanced splicing of XBP-1 was observed in BCL-2 overexpressing cells implicating BCL-2 in the complex regulation of IRE1α activity.
(Copyright © 2015 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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