| Autor: |
Tenje M; Department of Biomedical Engineering, Lund University, Lund, Sweden, maria.tenje@angstrom.uu.se., Lundgren MN, Swärd-Nilsson AM, Kjeldsen-Kragh J, Lyxe L, Lenshof A |
| Jazyk: |
angličtina |
| Zdroj: |
Biomedical microdevices [Biomed Microdevices] 2015 Oct; Vol. 17 (5), pp. 95. |
| DOI: |
10.1007/s10544-015-0003-5 |
| Abstrakt: |
This work presents the development of a miniaturized system for removing plasma proteins and other low-molecular-weight compounds from red blood cell (RBC) concentrate in a simple one-step-process using integrated ultrasound. The technology utilizes the principles of acoustophoresis to transfer the RBCs from the original plasma-containing solution into a protein-free SAG-M additive solution in a continuous flow process. The preparation of protein free RBC concentrate is important for blood transfusion to patients suffering from immunoglobulin A (IgA)-deficiency and developing antibodies against IgA. We show a nearly complete removal of both albumin and IgA from concentrated RBCs via this one-step-processes in samples obtained from RBC concentrate. The cell recovery of our technology is close to 97%, compared to just above 90% of the current procedure of repeated dilution and centrifugation steps. This work clearly shows the potential of integrated acoustophoresis in a miniaturized system for clinical applications. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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