[Effects of Garcinia Acid Combined with Daunorubicin on Expression of Pregnane X Receptor in Leukemia Cell Line K562/A02].

Autor:	Zhu H; Department of Hematology (Key Department of Jiangsu Province), Zhongda Hospital, Medical School, Southeast University, Nanjing 210009, Jiangsu Province, China., Chen BA; Department of Hematology (Key Department of Jiangsu Province), Zhongda Hospital, Medical School, Southeast University, Nanjing 210009, Jiangsu Province, China. E-mail: cba8888@hotmail.com., Cai XH; Department of Hematology (Key Department of Jiangsu Province), Zhongda Hospital, Medical School, Southeast University, Nanjing 210009, Jiangsu Province, China., Wang F; Department of Hematology (Key Department of Jiangsu Province), Zhongda Hospital, Medical School, Southeast University, Nanjing 210009, Jiangsu Province, China., Gao C; Department of Hematology (Key Department of Jiangsu Province), Zhongda Hospital, Medical School, Southeast University, Nanjing 210009, Jiangsu Province, China., Cheng J; Department of Hematology (Key Department of Jiangsu Province), Zhongda Hospital, Medical School, Southeast University, Nanjing 210009, Jiangsu Province, China., Zhang XP; Department of Hematology (Key Department of Jiangsu Province), Zhongda Hospital, Medical School, Southeast University, Nanjing 210009, Jiangsu Province, China., Dai L; Department of Hematology (Key Department of Jiangsu Province), Zhongda Hospital, Medical School, Southeast University, Nanjing 210009, Jiangsu Province, China., Xia GH; Department of Hematology (Key Department of Jiangsu Province), Zhongda Hospital, Medical School, Southeast University, Nanjing 210009, Jiangsu Province, China., Fu R; Jiangsu Key Laboratory of Carcinogenesis and Intervention, China Pharmaceutical University, Nanjing 210009, Jiangsu Province, China., Guo QL; Jiangsu Key Laboratory of Carcinogenesis and Intervention, China Pharmaceutical University, Nanjing 210009, Jiangsu Province, China.
Jazyk:	čínština
Zdroj:	Zhongguo shi yan xue ye xue za zhi [Zhongguo Shi Yan Xue Ye Xue Za Zhi] 2015 Aug; Vol. 23 (4), pp. 956-61.
DOI:	10.7534/j.issn.1009-2137.2015.04.009
Abstrakt:	Objective: To explore the expression of PXR (Pregnane X receptor) in several malignant hematological cell lines, and to investigate the reversal effect of Gambogic acid (GA) on multi-drug resistance (MDR) of K562/A02 cell line and its reversal mechanism. Methods: Transcription of PXR was detected by real-time PCR in several malignant hematological cell lines. The growth inhibition rate of K562/A02 in different experimental groups was assayed by MTT method, and the expression of PXR protein was measured by Western blot. Results: PXR gene transcription could be detected in several hematological malignancy cell lines, and it was significantly higher in K562/A02 cell line, compared with the other cell lines used in this experiment. Low-dose GA could enhance cell growth inhibition rate, increasing the effect of chemotherapy, which may be associated with down-regulation of PXR expression. PXR gene transcription and protein expression in GA and DNR+GA groups decreased as compared with control group and the DNR group, suggesting that low-dose GA can down-regulate PXR gene transcription and protein expression. Conclusion: PXR gene transcription can be detected in several hematological malignancy cell line, which is significantly higher in K562/A02 cell line, as compared with the other cell lines used in this experiment. Low-dose GA can enhance cell growth inhibition rate, increasing the effect of chemotherapy, which may be associated with down-regulation of PXR expression.
Databáze:	MEDLINE
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