Scavenger Receptor Class B Type I Regulates Plasma Apolipoprotein E Levels and Dietary Lipid Deposition to the Liver.

Autor:	Karavia EA; Pharmacology Department, ‡Anatomy Histology and Embryology Department, §Medical Physics Department, and ∥Endocrinology Department, University of Patras Medical School , Rio Achaias, TK 26500, Greece., Papachristou NI; Pharmacology Department, ‡Anatomy Histology and Embryology Department, §Medical Physics Department, and ∥Endocrinology Department, University of Patras Medical School , Rio Achaias, TK 26500, Greece., Sakellaropoulos GC; Pharmacology Department, ‡Anatomy Histology and Embryology Department, §Medical Physics Department, and ∥Endocrinology Department, University of Patras Medical School , Rio Achaias, TK 26500, Greece., Xepapadaki E; Pharmacology Department, ‡Anatomy Histology and Embryology Department, §Medical Physics Department, and ∥Endocrinology Department, University of Patras Medical School , Rio Achaias, TK 26500, Greece., Papamichail E; Pharmacology Department, ‡Anatomy Histology and Embryology Department, §Medical Physics Department, and ∥Endocrinology Department, University of Patras Medical School , Rio Achaias, TK 26500, Greece., Petropoulou PI; Pharmacology Department, ‡Anatomy Histology and Embryology Department, §Medical Physics Department, and ∥Endocrinology Department, University of Patras Medical School , Rio Achaias, TK 26500, Greece., Papakosta EP; Pharmacology Department, ‡Anatomy Histology and Embryology Department, §Medical Physics Department, and ∥Endocrinology Department, University of Patras Medical School , Rio Achaias, TK 26500, Greece., Constantinou C; Pharmacology Department, ‡Anatomy Histology and Embryology Department, §Medical Physics Department, and ∥Endocrinology Department, University of Patras Medical School , Rio Achaias, TK 26500, Greece., Habeos I; Pharmacology Department, ‡Anatomy Histology and Embryology Department, §Medical Physics Department, and ∥Endocrinology Department, University of Patras Medical School , Rio Achaias, TK 26500, Greece., Papachristou DJ; Pharmacology Department, ‡Anatomy Histology and Embryology Department, §Medical Physics Department, and ∥Endocrinology Department, University of Patras Medical School , Rio Achaias, TK 26500, Greece., Kypreos KE; Pharmacology Department, ‡Anatomy Histology and Embryology Department, §Medical Physics Department, and ∥Endocrinology Department, University of Patras Medical School , Rio Achaias, TK 26500, Greece.
Jazyk:	angličtina
Zdroj:	Biochemistry [Biochemistry] 2015 Sep 15; Vol. 54 (36), pp. 5605-16. Date of Electronic Publication: 2015 Sep 03.
DOI:	10.1021/acs.biochem.5b00700
Abstrakt:	Scavenger receptor class B type I (SR-BI) is primarily responsible for the selective uptake of cholesteryl esters (CE) of high-density lipoprotein (HDL) by the liver and other tissues. In the present study, we show that SR-BI-deficient (scarb1(-/-)) mice are resistant to diet-induced obesity, hepatic lipid deposition, and glucose intolerance after 24 weeks of being fed a western-type diet. No differences in energy expenditure or mitochondrial function could account for the observed phenotype. Kinetic and gene expression analyses suggested reduced de novo fatty acid synthesis in scarb1(-/-) mice. Furthermore, adenosine monophosphate-activated protein kinase (AMPK)-stimulated hepatic FFA catabolism was reduced in these mice, leaving direct dietary lipid uptake from plasma as the major modulator of hepatic lipid content. Analysis of the apolipoprotein composition of plasma lipoproteins revealed a significant accumulation of apolipoprotein E (ApoE)-containing HDL and TG-rich lipoproteins in scarb1(-/-) mice that correlated with reduced plasma LpL activity. Our data suggest that scarb1(-/-) mice fed a western-type diet for 24 weeks accumulate CE- and ApoE-rich HDL of abnormal density and size. The elevated HDL-ApoE levels inhibit plasma LpL activity, blocking the clearance of triglyceride-rich lipoproteins and preventing the shuttling of dietary lipids to the liver.
Databáze:	MEDLINE
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