Conditional Ultrasound Sensitivity of Poly[(N-isopropylacrylamide)-co-(vinyl imidazole)] Microgels for Controlled Lipase Release.

Autor: Vukićević R; Institute of Biomaterial Science, Helmholtz-Zentrum Geesthacht, Kantstraße 55, 14513, Teltow, Germany., Neffe AT; Institute of Biomaterial Science, Helmholtz-Zentrum Geesthacht, Kantstraße 55, 14513, Teltow, Germany.; Berlin-Brandenburg Center for Regenerative Therapies, Kanststr. 55, 14513, Teltow, Germany.; Institute of Chemistry, University of Potsdam, Karl-Liebknecht-Straße 24-25, 14476, Potsdam, Germany., Luetzow K; Institute of Biomaterial Science, Helmholtz-Zentrum Geesthacht, Kantstraße 55, 14513, Teltow, Germany., Pierce BF; Institute of Biomaterial Science, Helmholtz-Zentrum Geesthacht, Kantstraße 55, 14513, Teltow, Germany.; Berlin-Brandenburg Center for Regenerative Therapies, Kanststr. 55, 14513, Teltow, Germany., Lendlein A; Institute of Biomaterial Science, Helmholtz-Zentrum Geesthacht, Kantstraße 55, 14513, Teltow, Germany.; Berlin-Brandenburg Center for Regenerative Therapies, Kanststr. 55, 14513, Teltow, Germany.; Institute of Chemistry, University of Potsdam, Karl-Liebknecht-Straße 24-25, 14476, Potsdam, Germany.
Jazyk: angličtina
Zdroj: Macromolecular rapid communications [Macromol Rapid Commun] 2015 Nov; Vol. 36 (21), pp. 1891-1896. Date of Electronic Publication: 2015 Aug 27.
DOI: 10.1002/marc.201500311
Abstrakt: Triggering the release of cargo from a polymer network by ultrasonication as an external, noninvasive stimulus can be an interesting concept for on-demand release. Here, it is shown that, in pH- and thermosensitive microgels, the ultrasound sensitivity of the polymer network depends on the external conditions. Crosslinked poly[(N-isopropylacrylamide)-co-(vinyl imidazole)] microgels showed a volume phase transition temperature (VPTT) of 25-50 °C, which increases with decreasing pH. Above the VPTT the polymer chains are collapsed, while below VPTT they are extended. Only in the case of maximum observed swelling, where the polymer chains are expanded, the microgels are mechanically fragmented through ultrasonication. In contrast, when the polymer chains are partially collapsed it is not possible to manipulate the microgels by ultrasound. Additionally, the ultrasound-induced on-demand release of wheat germ lipase from the microgels could be demonstrated successfully. The principle of conditional ultrasound sensitivity is likely to be general and can be used for selection of matrix-cargo combinations.
(© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
Databáze: MEDLINE
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