Labeling of Enveloped Virus via Metabolic Incorporation of Azido Sugars.

Autor:	Zhao X; State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences , Changchun 130022, P. R. China., Cai L; Division of Mathematics and Science, University of South Carolina Salkehatchie , Walterboro, South Carolina 29488, United States., Adogla EA; Department of Chemistry and Biochemistry, University of South Carolina , Columbia, South Carolina 29208, United States., Guan H; Department of Chemistry and Biochemistry, University of South Carolina , Columbia, South Carolina 29208, United States., Lin Y; State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences , Changchun 130022, P. R. China., Wang Q; Department of Chemistry and Biochemistry, University of South Carolina , Columbia, South Carolina 29208, United States.
Jazyk:	angličtina
Zdroj:	Bioconjugate chemistry [Bioconjug Chem] 2015 Sep 16; Vol. 26 (9), pp. 1868-72. Date of Electronic Publication: 2015 Sep 03.
DOI:	10.1021/acs.bioconjchem.5b00310
Abstrakt:	Modification of an enveloped measles virus was achieved by metabolic incorporation of azido sugars in host cells through the protein glycosylation process. Based on this, the resulting measles virus particles could be modified with azido groups on the surface glycoproteins, which could be further labeled with fluorescence dyes using a strain-promoted azide-alkyne cycloaddition reaction. We envision this metabolic labeling approach to be applicable to a wide variety of enveloped viruses, allowing the facile conjugation and surface modification.
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu