Highly stable multi-anchored magnetic nanoparticles for optical imaging within biofilms.

Autor: Stone RC; Clemson University, Department of Materials Science and Engineering and Center for Optical Materials Science and Engineering Technologies (COMSET), 91 Technology Drive, Anderson, SC 29625, USA., Fellows BD; Clemson University, Department of Materials Science and Engineering and Center for Optical Materials Science and Engineering Technologies (COMSET), 91 Technology Drive, Anderson, SC 29625, USA., Qi B; Clemson University, Department of Materials Science and Engineering and Center for Optical Materials Science and Engineering Technologies (COMSET), 91 Technology Drive, Anderson, SC 29625, USA., Trebatoski D; University of Wisconsin-Madison, Materials Science and Engineering, 1509 University Avenue, Madison, WI 53706, USA., Jenkins B; Clemson University, Department of Biological Sciences, 132 Long Hall, Clemson, SC 29634, USA., Raval Y; Clemson University, Department of Biological Sciences, 132 Long Hall, Clemson, SC 29634, USA., Tzeng TR; Clemson University, Department of Biological Sciences, 132 Long Hall, Clemson, SC 29634, USA., Bruce TF; Clemson University, Department of Biological Sciences, 132 Long Hall, Clemson, SC 29634, USA; Clemson University, Light Imaging Facility, 190 Collings Street, Clemson, SC 29634, USA., McNealy T; Clemson University, Department of Biological Sciences, 132 Long Hall, Clemson, SC 29634, USA; Clemson University Institute of Environmental Toxicology (CU-ENTOX), 509 Westinghouse Road, Pendleton, SC 29670, USA., Austin MJ; Sandia National Laboratories, Albuquerque, NM 87185, USA., Monson TC; Sandia National Laboratories, Albuquerque, NM 87185, USA., Huber DL; Sandia National Laboratories, Albuquerque, NM 87185, USA., Mefford OT; Clemson University, Department of Materials Science and Engineering and Center for Optical Materials Science and Engineering Technologies (COMSET), 91 Technology Drive, Anderson, SC 29625, USA; Clemson University Institute of Environmental Toxicology (CU-ENTOX), 509 Westinghouse Road, Pendleton, SC 29670, USA. Electronic address: mefford@clemson.edu.
Jazyk: angličtina
Zdroj: Journal of colloid and interface science [J Colloid Interface Sci] 2015 Dec 01; Vol. 459, pp. 175-182. Date of Electronic Publication: 2015 Aug 05.
DOI: 10.1016/j.jcis.2015.08.012
Abstrakt: Magnetic nanoparticles are the next tool in medical diagnoses and treatment in many different biomedical applications, including magnetic hyperthermia as alternative treatment for cancer and bacterial infections, as well as the disruption of biofilms. The colloidal stability of the magnetic nanoparticles in a biological environment is crucial for efficient delivery. A surface that can be easily modifiable can also improve the delivery and imaging properties of the magnetic nanoparticle by adding targeting and imaging moieties, providing a platform for additional modification. The strategy presented in this work includes multiple nitroDOPA anchors for robust binding to the surface tied to the same polymer backbone as multiple poly(ethylene oxide) chains for steric stability. This approach provides biocompatibility and enhanced stability in fetal bovine serum (FBS) and phosphate buffer saline (PBS). As a proof of concept, these polymer-particles complexes were then modified with a near infrared dye and utilized in characterizing the integration of magnetic nanoparticles in biofilms. The work presented in this manuscript describes the synthesis and characterization of a nontoxic platform for the labeling of near IR-dyes for bioimaging.
(Copyright © 2015 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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