Research using Mesenchymal Stem/Stromal Cells: quality metric towards developing a reference material.

Autor: Tanavde V; Bioinformatics Institute, Agency for Science Technology and Research (A*STAR), Singapore 138671; Institute for Medical Biology, A∗STAR, Singapore 138648., Vaz C; Bioinformatics Institute, Agency for Science Technology and Research (A*STAR), Singapore 138671., Rao MS; Q Thera, NYSCF, Regenerative Medicine, NYSTEM, Albany, NY., Vemuri MC; Thermo Fisher Scientific, Frederick, MD 21704, USA., Pochampally RR; Cancer Institute, University of Mississippi Medical Center, Jackson, MS 39216, USA. Electronic address: rpochampally@umc.edu.
Jazyk: angličtina
Zdroj: Cytotherapy [Cytotherapy] 2015 Sep; Vol. 17 (9), pp. 1169-77.
DOI: 10.1016/j.jcyt.2015.07.008
Abstrakt: Mesenchymal stem/stromal cells (MSCs) have been extensively investigated for their regenerative, immune-modulatory, and wound healing properties. While the laboratory studies have suggested that MSC's have a unique potential for modulating the etiopathology of multiple diseases, the results from clinical trials have not been encouraging or reproducible. One of the explanations for such variability is explained by the "art" of isolating and propagating MSCs. Therefore, establishing more than minimal criteria to define MSC would help understand best protocols to isolate, propagate and deliver MSCs. Developing a calibration standard, a database and a set of functional tests would be a better quality metric for MSCs. In this review, we discuss the importance of selecting a standard, issues associated with coming up with such a standard and how these issues can be mitigated.
(Copyright © 2015 International Society for Cellular Therapy. Published by Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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