| Autor: |
Oliveira KG; Department of Gastroenterology, University of São Paulo School of Medicine, São Paulo, Brazil.; Hospital Israelita Albert Einstein, São Paulo, Brazil., Malta FM; Department of Gastroenterology, University of São Paulo School of Medicine, São Paulo, Brazil. femalta@yahoo.com.; Institute of Tropical Medicine, University of São Paulo, Av. Dr. Enéas Carvalho Aguiar, 500-2 ̊ andar-IMT II, São Paulo, SP, 05403-000, Brazil. femalta@yahoo.com., Nastri AC; Department of Infectious and Parasitic Diseases, University of São Paulo, School of Medicine, São Paulo, Brazil., Widman A; Department of Gastroenterology, University of São Paulo School of Medicine, São Paulo, Brazil., Faria PL; Department of Gastroenterology, University of São Paulo School of Medicine, São Paulo, Brazil.; Institute of Tropical Medicine, University of São Paulo, Av. Dr. Enéas Carvalho Aguiar, 500-2 ̊ andar-IMT II, São Paulo, SP, 05403-000, Brazil., Santana RA; Hospital Israelita Albert Einstein, São Paulo, Brazil., Alves VA; Department of Pathology, University of São Paulo School of Medicine, São Paulo, Brazil., Carrilho FJ; Department of Gastroenterology, University of São Paulo School of Medicine, São Paulo, Brazil., Pinho JR; Department of Gastroenterology, University of São Paulo School of Medicine, São Paulo, Brazil.; Institute of Tropical Medicine, University of São Paulo, Av. Dr. Enéas Carvalho Aguiar, 500-2 ̊ andar-IMT II, São Paulo, SP, 05403-000, Brazil.; Hospital Israelita Albert Einstein, São Paulo, Brazil. |
| Abstrakt: |
Hepatitis C virus (HCV) infection affects approximately 3 % of the world population. HCV targets hepatic tissue, and most infected patients develop a chronic infection. Currently, studies have demonstrated an association between HCV-RNA replication and miR-122, the most abundant microRNA in the liver. Our aim was to evaluate liver and serum expression of miR-122 in patients infected with HCV genotypes 1 and 3, and to identify possible associations between miR-122 expression and lipid profiles, HCV viral load, apolipoproteins and liver enzymes. MicroRNAs were isolated from blood and liver tissue, and miR-122 expression was quantified by real-time PCR. HCV viral load was quantified by real-time PCR and HCV genotype, and serum biomarkers were obtained from medical report. The levels of miR-122 were higher in liver than those in blood from individuals infected with HCV genotypes 1 and 3 (p < 0.0001). The tissue levels of miR-122 were higher in subjects infected with HCV genotype 3 (6.22-fold, p < 0.001). A positive correlation was observed between the blood and hepatic levels of miR-122 in patients infected with HCV genotype 1 (r = 0.302, p = 0.026); in these patients, an inverse correlation was observed between serum apolipoprotein A-II (ApoA-II) levels and the blood (r = -0.330; p = 0.014) and hepatic (r = -0.311; p = 0.020) levels of miR-122. In patients infected with HCV genotype 3, there was a positive correlation between the hepatic miR-122 and the high-density lipoprotein-HDL (r = 0.412, p = 0.036) and insulin (r = 0.478, p = 0.044). Lipid metabolism proteins and miR-122 expression levels have different relations in HCV-3- and HCV-1-infected patients. |
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