| Autor: |
Samico-Fernandes EF; Department of Veterinary Medicine, Laboratory of Infectious-Contagious Diseases of Domestic Animals, Universidade Federal Rural de Pernambuco, Rua Dom Manoel de Medeiros, Dois Irmãos, 52171-900, Recife, PE, Brazil., de Melo RP, de Cássia Peixoto Kim P, de Almeida JC, de Barros LD, Garcia JL, da Silva JC, Mota RA |
| Jazyk: |
angličtina |
| Zdroj: |
Parasitology research [Parasitol Res] 2015 Oct; Vol. 114 (10), pp. 3927-30. Date of Electronic Publication: 2015 Aug 14. |
| DOI: |
10.1007/s00436-015-4664-z |
| Abstrakt: |
The aim of the present study was to isolate and genotype Toxoplasma gondii from pigs slaughtered for human consumption in northeastern Brazil. Indirect immunofluorescence antibody test (IFAT) was used to screen positive pigs. Tissues samples of animals with antibody titers ≥64 were submitted to bioassay in mice. One isolate of T. gondii was obtained, and the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique, using 11 markers (SAG1, SAG2, altSAG2, SAG3, BTUB, GRA6, c228, c292, L358, PK1, and APICO), was applied to evaluate the genetic variability. DNA from reference strains was used as a positive control. By means of genetic analysis, genotype ToxoDB #65 was identified, which is considered an atypical strain. This is the first record of genotype #65 in pigs. Thus, further studies in this region are necessary to determine the genetic variability of T. gondii in pigs and possible impact on public health. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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