A conformational epitope mapped in the bovine herpesvirus type 1 envelope glycoprotein B by phage display and the HSV-1 3D structure.

Autor: Almeida GR; Departamento de Bioquímica e Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, GO, Brazil; Laboratório de Virologia Animal, Departamento de Microbiologia, Universidade Federal de Goiás, Goiânia, GO, Brazil. Electronic address: greyci_almeid@hotmail.com., Goulart LR; Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, Uberlândia, MG, Brazil. Electronic address: lrgoulart@ufu.br., Cunha-Junior JP; Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Uberlândia, MG, Brazil. Electronic address: jair.cunha.junior@gmail.com., Bataus LA; Departamento de Bioquímica e Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, GO, Brazil. Electronic address: bataus@ufg.br., Japolla G; Departamento de Bioquímica e Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, GO, Brazil; Laboratório de Virologia Animal, Departamento de Microbiologia, Universidade Federal de Goiás, Goiânia, GO, Brazil. Electronic address: gre_vet@hotmail.com., Brito WM; Laboratório de Virologia Animal, Departamento de Microbiologia, Universidade Federal de Goiás, Goiânia, GO, Brazil. Electronic address: wilia@hotmail.com., Campos IT; Departamento de Bioquímica e Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, GO, Brazil. Electronic address: ivan@icb.ufg.br., Ribeiro C; Departamento de Bioquímica e Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, GO, Brazil. Electronic address: cris.lino@gmail.com., Souza GR; Departamento de Bioquímica e Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, GO, Brazil. Electronic address: grlino@gmail.com.
Jazyk: angličtina
Zdroj: Research in veterinary science [Res Vet Sci] 2015 Aug; Vol. 101, pp. 34-7. Date of Electronic Publication: 2015 Jun 06.
DOI: 10.1016/j.rvsc.2015.05.021
Abstrakt: The selected dodecapeptide (1)DRALYGPTVIDH(12) from a phage-displayed peptide library and the crystal structure of the envelope glycoprotein B (Env gB) from Herpes Simplex Virus type 1 (HSV-1) led us to the identification of a new discontinuous epitope on the Bovine herpesvirus type 1 (BoHV-1) Env gB. In silico analysis revealed a short BoHV-1 gB motif ((338)YKRD(341)) within a epitope region, with a high similarity to the motifs shared by the dodecapeptide N-terminal region ((5)YxARD(1)) and HSV-1 Env gB ((326)YARD(329)), in which the (328)Arg residue is described to be a neutralizing antibody target. Besides the characterization of an antibody-binding site of the BoHV-1 Env gB, we have demonstrated that the phage-fused peptide has the potential to be used as a reagent for virus diagnosis by phage-ELISA assay, which discriminated BoHV-1 infected serum samples from negative ones.
(Copyright © 2015 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: