Acute organ injury is associated with alterations in the cell-free plasma transcriptome.

Autor: Boyd JH; Centre for Heart Lung Innovation, St. Paul's Hospital, Room 166, 1081 Burrard Street, Vancouver, British Columbia, V6Z 1Y6, Canada, John.Boyd@hli.ubc.ca., McConechy M, Walley KR
Jazyk: angličtina
Zdroj: Intensive care medicine experimental [Intensive Care Med Exp] 2014 Dec; Vol. 2 (1), pp. 7. Date of Electronic Publication: 2014 Feb 21.
DOI: 10.1186/2197-425X-2-7
Abstrakt: Background: Despite a genomic revolution in biological sciences, clinical medicine has yet to integrate diagnostics based upon gene expression into practice. While commonly used plasma protein assays rely on organ-specific origins, nearly all nucleic acid in whole blood is derived from white blood cells limiting their utility to diagnose non-immune disorders. The aim of the study was to use cell-free plasma to define circulating messenger RNA sequences diagnostic of acute organ injury, including myocardial infarction (MI) and acute kidney injury (AKI).
Methods: In healthy human subjects (N = 4) and patients with acute MI (N = 4), we characterized the concentration and nature of circulating plasma RNA through spectrophotometry and chromatography. Through reverse transcriptase polymerase chain reaction (RT-PCR) of amplicons up to 939 base pairs, we determined whether this mRNA was intact but of insufficient quantity to sequence. In mice, we induced an acute anterior myocardial infarction through 1 h of ischemia followed by reperfusion of the left anterior descending (LAD) artery. We compared the cell-free plasma transcriptome using cDNA microarray in sham-operated mice compared to ischemia upon reperfusion and at 1 and 4 h. To determine organ specificity, we compared this profile to acute ischemia-reperfusion of the kidney.
Results: In humans, there is more plasma RNA in those with acute MI than in healthy controls. In mice, ischemia-reperfusion of the LAD artery resulted in a time-dependent regulation of 589 circulating mRNA transcripts with less than a 5% overlap in sequences from acute ischemia-reperfusion injury of the kidney.
Conclusions: The mRNA derived from cell-free plasma defines organ injury in a time and injury-specific pattern.
Databáze: MEDLINE
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