A new heterologous fibrin sealant as a scaffold to cartilage repair-Experimental study and preliminary results.

Autor:	de Barros CN; Department of Veterinary Surgery and Anesthesiology, School of Veterinary Medicine and Animal Science, São Paulo State University (UNESP-Univ Estadual Paulista), Rubião District, 18618-970 Botucatu, São Paulo, Brazil., Miluzzi Yamada AL; Department of Veterinary Surgery and Anesthesiology, School of Veterinary Medicine and Animal Science, São Paulo State University (UNESP-Univ Estadual Paulista), Rubião District, 18618-970 Botucatu, São Paulo, Brazil., Junior RS; Center for the Study of Venoms and Venomous Animals, São Paulo State University (UNESP-Univ Estadual Paulista), Rubião District, 18618-970 Botucatu, São Paulo, Brazil., Barraviera B; Center for the Study of Venoms and Venomous Animals, São Paulo State University (UNESP-Univ Estadual Paulista), Rubião District, 18618-970 Botucatu, São Paulo, Brazil., Hussni CA; Department of Veterinary Surgery and Anesthesiology, School of Veterinary Medicine and Animal Science, São Paulo State University (UNESP-Univ Estadual Paulista), Rubião District, 18618-970 Botucatu, São Paulo, Brazil., de Souza JB; Department of Veterinary Surgery and Anesthesiology, School of Veterinary Medicine and Animal Science, São Paulo State University (UNESP-Univ Estadual Paulista), Rubião District, 18618-970 Botucatu, São Paulo, Brazil., Watanabe MJ; Department of Veterinary Surgery and Anesthesiology, School of Veterinary Medicine and Animal Science, São Paulo State University (UNESP-Univ Estadual Paulista), Rubião District, 18618-970 Botucatu, São Paulo, Brazil., Rodrigues CA; Department of Veterinary Surgery and Anesthesiology, School of Veterinary Medicine and Animal Science, São Paulo State University (UNESP-Univ Estadual Paulista), Rubião District, 18618-970 Botucatu, São Paulo, Brazil., Garcia Alves AL; Department of Veterinary Surgery and Anesthesiology, School of Veterinary Medicine and Animal Science, São Paulo State University (UNESP-Univ Estadual Paulista), Rubião District, 18618-970 Botucatu, São Paulo, Brazil anaalves@fmvz.unesp.br.
Jazyk:	angličtina
Zdroj:	Experimental biology and medicine (Maywood, N.J.) [Exp Biol Med (Maywood)] 2016 Jul; Vol. 241 (13), pp. 1410-5. Date of Electronic Publication: 2015 Aug 10.
DOI:	10.1177/1535370215597192
Abstrakt:	Autologous fibrin gel is commonly used as a scaffold for filling defects in articular cartilage. This biomaterial can also be used as a sealant to control small hemorrhages and is especially helpful in situations where tissue reparation capacity is limited. In particular, fibrin can act as a scaffold for various cell types because it can accommodate cell migration, differentiation, and proliferation. Despite knowledge of the advantages of this biomaterial and mastery of the techniques required for its application, the durability of several types of sealant at the site of injury remains questionable. Due to the importance of such data for evaluating the quality and efficiency of fibrin gel formulations on its use as a scaffold, this study sought to analyze the heterologous fibrin sealant developed from the venom of Crotalus durissus terrificus using studies in ovine experimental models. The fibrin gel developed from the venom of this snake was shown to act as a safe, stable, and durable scaffold for up to seven days, without causing adverse side effects. Fibrin gel produced from the venom of the Crotalus durissus terrificus snake possesses many clinical and surgical uses. It presents the potential to be used as a biomaterial to help repair skin lesions or control bleeding, and it may also be used as a scaffold when applied together with various cell types. The intralesional use of the fibrin gel from the venom of this snake may improve surgical and clinical treatments in addition to being inexpensive and adequately consistent, durable, and stable. The new heterologous fibrin sealant is a scaffold candidate to cartilage repair in this study. (© 2015 by the Society for Experimental Biology and Medicine.)
Databáze:	MEDLINE
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