Efficiency of photodynamic therapy on WM35 melanoma with synthetic porphyrins: Role of chemical structure, intracellular targeting and antioxidant defense.

Autor: Baldea I; University of Medicine and Pharmacy, Department of Physiology, Clinicilor 1, Cluj-Napoca, Romania. Electronic address: baldeai@yahoo.com., Olteanu DE; University of Medicine and Pharmacy, Department of Physiology, Clinicilor 1, Cluj-Napoca, Romania. Electronic address: ariana_di@yahoo.com., Bolfa P; University of Agricultural Sciences and Veterinary Medicine, Department of Pathology, Calea Manastur 3-5, 400372 Cluj-Napoca, Romania; Ross University School of Veterinary Medicine, Department of Biomedical Sciences, PO Box 334, Basseterre, Saint Kitts and Nevis. Electronic address: pompei.bolfa@usamvcluj.ro., Ion RM; National Institute for Research & Development in Chemistry and Petrochemistry - ICECHIM, Nanomedicine Research Group, 202 Splaiul Independentei, Sector 6, Bucharest, Romania. Electronic address: rodicaion2000@yahoo.co.uk., Decea N; University of Medicine and Pharmacy, Department of Physiology, Clinicilor 1, Cluj-Napoca, Romania. Electronic address: nicoleta_decea@yahoo.com., Cenariu M; University of Agricultural Sciences and Veterinary Medicine, Department of Biochemistry, Calea Manastur 3-5, 400372 Cluj-Napoca, Romania. Electronic address: mihai.cenariu@usamvcluj.ro., Banciu M; Department of Molecular Biology and Biotechnology, Faculty of Biology and Geology, Babeş-Bolyai University, Clinicilor Street 5-7, 400006 Cluj-Napoca, Romania. Electronic address: manuela.banciu@ubbcluj.ro., Sesarman AV; Department of Molecular Biology and Biotechnology, Faculty of Biology and Geology, Babeş-Bolyai University, Clinicilor Street 5-7, 400006 Cluj-Napoca, Romania. Electronic address: alina.sesarman@hasdeu.ubbcluj.ro., Filip AG; University of Medicine and Pharmacy, Department of Physiology, Clinicilor 1, Cluj-Napoca, Romania. Electronic address: adrianafilip33@yahoo.com.
Jazyk: angličtina
Zdroj: Journal of photochemistry and photobiology. B, Biology [J Photochem Photobiol B] 2015 Oct; Vol. 151, pp. 142-52. Date of Electronic Publication: 2015 Jul 29.
DOI: 10.1016/j.jphotobiol.2015.07.019
Abstrakt: Photodynamic therapy (PDT) could be an adjuvant therapy in melanoma, an aggressive cancer that arises from melanocytes. Several reports showed encouraging results of the efficacy of PDT in melanoma on experimental models and in clinical trials. Therefore, we studied the efficacy of two derivatives of tetraphenylporphyrin (TPP): meso-5,10,15,20-tetrakis (4-hydroxyphenyl) porphyrin (THOPP) and meso-5-(4-hydroxyphenyl)-10,15,20-tris (4-methoxyphenyl) porphyrin (THOMPP) as photosensitizers for PDT, compared to FDA approved delta aminolevulinic acid (ALA) against a lightly pigmented, melanoma cell line, WM35, in vitro. Both porphyrins were more efficient as photosensitizers, compared to ALA, without dark toxicity. The efficiency depended on the intracellular localization and the molecule structure. THOPP, the most efficient porphyrin localized mainly in mitochondria, while THOMPP accumulated in lysosomes; both showed melanosomal localization. The symmetric THOPP molecule was able to generate increased oxidative stress damage and apoptosis. THOPP also induced a low effect on the defense mechanisms like antioxidant enzyme SOD (superoxide dismutase), NF-kB (nuclear transcription factor kB) activation and MITF (microphthalmia transcription factor). The lower efficiency of the asymmetric molecule, THOMPP was probably due to a diminished photoactivation, which led to a lower ROS induced damage, combined with higher activation of the defense mechanisms.
(Copyright © 2015 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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