Accumulation of recalcitrant xylan in mushroom-compost is due to a lack of xylan substituent removing enzyme activities of Agaricus bisporus.

Autor: Jurak E; Wageningen University, Laboratory of Food Chemistry, Bornse Weilanden 9, 6708 WG Wageningen, The Netherlands. Electronic address: edita.jurak@wur.nl., Patyshakuliyeva A; CBS-KNAW Fungal Biodiversity Centre, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands; Fungal Molecular Physiology, Utrecht University, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands. Electronic address: a.patyshakuliyeva@cbs.knaw.nl., Kapsokalyvas D; Department of Molecular Cell Biology, Cardiovascular Research Institute Maastricht (CARIM), Maastricht University, Maastricht, The Netherlands. Electronic address: d.kapsokalyvas@maastrichtuniversity.nl., Xing L; Wageningen University, Laboratory of Food Chemistry, Bornse Weilanden 9, 6708 WG Wageningen, The Netherlands. Electronic address: lia.xing90@gmail.com., van Zandvoort MA; Department of Molecular Cell Biology, Cardiovascular Research Institute Maastricht (CARIM), Maastricht University, Maastricht, The Netherlands; Institute for Molecular Cardiovascular Research (IMCAR), RWTH Aachen University, Aachen, Germany. Electronic address: mamj.vanzandvoort@maastrichtuniversity.nl., de Vries RP; CBS-KNAW Fungal Biodiversity Centre, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands; Fungal Molecular Physiology, Utrecht University, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands. Electronic address: r.devries@cbs.knaw.nl., Gruppen H; Wageningen University, Laboratory of Food Chemistry, Bornse Weilanden 9, 6708 WG Wageningen, The Netherlands. Electronic address: harry.gruppen@wur.nl., Kabel MA; Wageningen University, Laboratory of Food Chemistry, Bornse Weilanden 9, 6708 WG Wageningen, The Netherlands. Electronic address: mirjam.kabel@wur.nl.
Jazyk: angličtina
Zdroj: Carbohydrate polymers [Carbohydr Polym] 2015 Nov 05; Vol. 132, pp. 359-68. Date of Electronic Publication: 2015 Jun 26.
DOI: 10.1016/j.carbpol.2015.06.065
Abstrakt: The ability of Agaricus bisporus to degrade xylan in wheat straw based compost during mushroom formation is unclear. In this paper, xylan was extracted from the compost with water, 1M and 4M alkali. Over the phases analyzed, the remaining xylan was increasingly substituted with (4-O-methyl-)glucuronic acid and arabinosyl residues, both one and two arabinosyl residues per xylosyl residue remained. In the 1M and 4M KOH soluble solids of spent compost, 33 and 49 out of 100 xylosyl residues, respectively, were substituted. The accumulation of glucuronic acid substituents matched with the analysis that the two A. bisporus genes encoding for α-glucuronidase activity (both GH115) were not expressed in the A. bisporus mycelium in the compost during fruiting. Also, in a maximum likelihood tree it was shown that it is not likely that A. bisporus possesses genes encoding for the activity to remove arabinose from xylosyl residues having two arabinosyl residues.
(Copyright © 2015 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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