| Autor: |
Machado BA; Institute of Research and Technology, Tiradentes University, Aracaju, Sergipe, Brazil; Faculty of Technology, SENAI/CIMATEC, National Service of Industrial Learning-SENAI, Salvador, Bahia, Brazil., Barreto Gde A; Faculty of Technology, SENAI/CIMATEC, National Service of Industrial Learning-SENAI, Salvador, Bahia, Brazil., Costa AS; Faculty of Technology, SENAI/CIMATEC, National Service of Industrial Learning-SENAI, Salvador, Bahia, Brazil., Costa SS; Faculty of Technology, SENAI/CIMATEC, National Service of Industrial Learning-SENAI, Salvador, Bahia, Brazil., Silva RP; Faculty of Technology, SENAI/CIMATEC, National Service of Industrial Learning-SENAI, Salvador, Bahia, Brazil., da Silva DF; Faculty of Pharmacy, State University of Feira de Santana, Feira de Santana, Bahia, Brazil., Brandão HN; Faculty of Pharmacy, State University of Feira de Santana, Feira de Santana, Bahia, Brazil., da Rocha JL; Faculty of Pharmacy, State University of Feira de Santana, Feira de Santana, Bahia, Brazil., Nunes SB; Faculty of Technology, SENAI/CIMATEC, National Service of Industrial Learning-SENAI, Salvador, Bahia, Brazil., Umsza-Guez MA; Biotechnology, Federal University of Bahia, Salvador, Bahia, Brazil., Padilha FF; Institute of Research and Technology, Tiradentes University, Aracaju, Sergipe, Brazil. |
| Abstrakt: |
The aim of this study was to determine the best processing conditions to extract Brazilian green propolis using a supercritical extraction technology. For this purpose, the influence of different parameters was evaluated such as S/F (solvent mass in relation to solute mass), percentage of co-solvent (1 and 2% ethanol), temperature (40 and 50°C) and pressure (250, 350 and 400 bar) using supercritical carbon dioxide. The Global Yield Isotherms (GYIs) were obtained through the evaluation of the yield, and the chemical composition of the extracts was also obtained in relation to the total phenolic compounds, flavonoids, antioxidant activity and 3,5-diprenyl-4-hydroxicinnamic acid (Artepillin C) and acid 4-hydroxycinnamic (p-coumaric acid). The best results were identified at 50°C, 350 bar, 1% ethanol (co-solvent) and S/F of 110. These conditions, a content of 8.93±0.01 and 0.40±0.05 g/100 g of Artepillin C and p-coumaric acid, respectively, were identified indicating the efficiency of the extraction process. Despite of low yield of the process, the extracts obtained had high contents of relevant compounds, proving the viability of the process to obtain green propolis extracts with important biological applications due to the extracts composition. |
