Evaluation of a collagen-coated, resorbable fiber scaffold loaded with a peptide basic fibroblast growth factor mimetic in a sheep model of rotator cuff repair.

Autor: Peterson DR; DRP Biomedical, Carlsbad, CA, USA. Electronic address: 2dalesmail@gmail.com., Ohashi KL; Microvascular Tissues, Boston, MA, USA., Aberman HM; Advanced Biological Concepts, Los Alamitos, CA, USA., Piza PA; Golden Orthopedic, Delray Beach, FL, USA., Crockett HC; New West Sports Medicine, Kearney, NE, USA., Fernandez JI; Miami Sports Medicine, Miami, FL, USA., Lund PJ; SimonMed Imaging, Scottsdale, AZ, USA., Funk KA; Experimental Pathology Laboratories, Sterling, VA, USA., Hawes ML; Charter Preclinical Services, Hudson, MA, USA., Parks BG; Union Memorial Hospital, Baltimore, MD, USA., Mattern RH; Microvascular Tissues, Boston, MA, USA.
Jazyk: angličtina
Zdroj: Journal of shoulder and elbow surgery [J Shoulder Elbow Surg] 2015 Nov; Vol. 24 (11), pp. 1764-73. Date of Electronic Publication: 2015 Aug 04.
DOI: 10.1016/j.jse.2015.06.009
Abstrakt: Background: A new scaffold design combined with a peptide growth factor was tested prospectively for safety and for improved tendon healing in sheep.
Methods: The infraspinatus tendon was detached and then surgically repaired to the humerus using sutures and anchors in 50 adult sheep. The repairs in 40 of these sheep were reinforced with a scaffold containing F2A, a peptide mimetic of basic fibroblast growth factor. The sheep were examined after 8 or 26 weeks with magnetic resonance imaging, full necropsy, and histopathologic analysis. A second cohort of 30 sheep underwent surgical repair--20 with scaffolds containing F2A. The 30 shoulders were tested mechanically after 8 weeks.
Results: The scaffold and F2A showed no toxicity. Scaffold-repaired tendons were 31% thicker than surgically repaired controls (P = .037) at 8 weeks. There was more new bone formed at the tendon footprint in sheep treated with F2A. Surgically repaired tendons delaminated from the humerus across 14% of the footprint area. The extent of delamination decreased to 1.3% with increasing doses of F2A (P = .004). More of the repair tissue at the footprint was tendon-like in the peptide-treated sheep. On mechanical testing, only 7 shoulders tore at the repair site. The repairs in the other 23 shoulders were already stronger than the midsubstance tendon at 8 weeks.
Conclusions: The new scaffold and peptide safely improved tendon healing.
(Copyright © 2015 Journal of Shoulder and Elbow Surgery Board of Trustees. Published by Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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