Assessment of pharmacokinetic interaction between piracetam and l-carnitine in healthy subjects.

Autor: Mendes GD; Department of Pharmacology, University Camilo Castelo Branco, SP, Brazil.; Galeno Research Unit, Latino Coelho St, 1301, Parque Taquaral, 13087-010, Campinas, SP, Brazil., Zaffalon GT; Department of Pharmacology, University Camilo Castelo Branco, SP, Brazil.; Faculty of Odontology, Dental Research Center São Leopoldo Mandic, Campinas, SP, Brazil., Silveira AS; Galeno Research Unit, Latino Coelho St, 1301, Parque Taquaral, 13087-010, Campinas, SP, Brazil., Ramacciato JC; Faculty of Odontology, Dental Research Center São Leopoldo Mandic, Campinas, SP, Brazil., Motta RH; Faculty of Odontology, Dental Research Center São Leopoldo Mandic, Campinas, SP, Brazil., Gagliano-Jucá T; Galeno Research Unit, Latino Coelho St, 1301, Parque Taquaral, 13087-010, Campinas, SP, Brazil., Lopes AG; Department of Physiology, Faculty of Medicine, University Camilo Castelo Branco, SP, Brazil., de Almeida Magalhães JC; Department of Pharmacology, University Camilo Castelo Branco, SP, Brazil., De Nucci G; Department of Pharmacology, University Camilo Castelo Branco, SP, Brazil.; Department of Pharmacology, University of Sao Paulo, São Paulo, SP, Brazil.
Jazyk: angličtina
Zdroj: Biomedical chromatography : BMC [Biomed Chromatogr] 2016 Apr; Vol. 30 (4), pp. 536-42. Date of Electronic Publication: 2015 Nov 04.
DOI: 10.1002/bmc.3579
Abstrakt: A rapid, sensitive and specific method for quantifying piracetam in human plasma using Piracetam d-8 as the internal standard (IS) is described. The analyte and the IS were extracted from plasma by one-step precipitation of protein using an acetonitrile (100%). The extracts were analyzed by high-performance liquid chromatography coupled with electrospray tandem mass spectrometry (HPLC-MS/MS). The method had a chromatographic run time of 3.8 min and a linear calibration curve over the range 0.5-50 µg/mL (r > 0.99). This LC-MS-MS procedure was used to assess the bioavailability of two piracetam formulations: piracetam + l-carnitine (Piracar®; 270/330 mg tablet) and piracetam (Nootropil®; 800 mg tablet) in healthy volunteers of both sexes. The geometric means with corresponding 90% confidence interval (CI) for test/reference percentage ratios were 88.49% (90% CI = 81.19 - 96.46) for peak concentration/dose and 102.55% (90% CI = 100.62 - 104.51) for AUCinf /dose. The limit of quantitation of 0.5 µg/mL is well suited for pharmacokinetic studies in healthy volunteers. It was concluded that piracetam (Piracar®; 270/330 mg tablet) has a bioavailability equivalent to the piracetam (Nootropil®; 800 mg tablet) formulation with regard to both the rate and the extent of absorption.
(Copyright © 2015 John Wiley & Sons, Ltd.)
Databáze: MEDLINE
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