[Effect of reactive oxygen species induced by paraquat on neutrophil apoptosis].
| Autor: | Qin KX, Li CW, Fang Y, Yu L, Wang XL |
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| Jazyk: | čínština |
| Zdroj: | Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology [Zhongguo Ying Yong Sheng Li Xue Za Zhi] 2015 Mar; Vol. 31 (2), pp. 111-4. |
| Abstrakt: | Objective: To investigate the effect of paraquat (PQ) on reactive oxygen species (ROS) and neutrophil apoptosis and its possible signal transduction pathways. Methods: Cultured neutrophils were treated with different concentrations of PQ for 6-24 h. The apoptosis rate of neutrophils and ROS content were determined by flow cytometry. The exoressions of nuclear factor kappa B (NF-κB) and Caspase 3 were detected by Western blot. These parameters were checked again after NF-κB and Caspase 3 antagonist were applied. Results: PQ could boost ROS generation and depress neutrophil apoptosis significantly. At the same time PQ could enhance the expression of NF-κB and inhibit the expression of Caspase 3. These effects could be reversed by ROS inhibitor diphenyleneiodonium (DPI) and NF-κB inhibitor pyrrolidinedithiocarbamate (PDTC). Conclusion: PQ is a potent inducer of ROS and can inhibit neutrophil apoptosis by activating NF-κB and surpressing Caspase 3 activity. |
| Databáze: | MEDLINE |
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