Identification of Genetic Mutations in Human Lung Cancer by Targeted Sequencing.

Autor:	Feng H; Department of Thoracic Surgery, China-Japan Friendship Hospital, Beijing, China., Wang X; Department of Thoracic Surgery, China-Japan Friendship Hospital, Beijing, China., Zhang Z; Department of Thoracic Surgery, China-Japan Friendship Hospital, Beijing, China., Tang C; San Valley Biotechnology Inc., Beijing, China., Ye H; San Valley Biotechnology Inc., Beijing, China., Jones L; Norris Comprehensive Cancer Center, Department of Molecular Microbiology and Immunology, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA., Lou F; San Valley Biotechnology Inc., Beijing, China., Zhang D; San Valley Biotechnology Inc., Beijing, China., Jiang S; San Valley Biotechnology Inc., Beijing, China., Sun H; San Valley Biotechnology Inc., Beijing, China., Dong H; San Valley Biotechnology Inc., Beijing, China., Zhang G; San Valley Biotechnology Inc., Beijing, China., Liu Z; San Valley Biotechnology Inc., Beijing, China., Dong Z; San Valley Biotechnology Inc., Beijing, China., Guo B; San Valley Biotechnology Inc., Beijing, China., Yan H; San Valley Biotechnology Inc., Beijing, China., Yan C; San Valley Biotechnology Inc., Beijing, China., Wang L; San Valley Biotechnology Inc., Beijing, China., Su Z; San Valley Biotechnology Inc., Beijing, China., Li Y; San Valley Biotechnology Inc., Beijing, China., Nandakumar V; Norris Comprehensive Cancer Center, Department of Molecular Microbiology and Immunology, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA., Huang XF; Norris Comprehensive Cancer Center, Department of Molecular Microbiology and Immunology, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA., Chen SY; Norris Comprehensive Cancer Center, Department of Molecular Microbiology and Immunology, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA., Liu D; Department of Thoracic Surgery, China-Japan Friendship Hospital, Beijing, China.
Jazyk:	angličtina
Zdroj:	Cancer informatics [Cancer Inform] 2015 Jun 29; Vol. 14, pp. 83-93. Date of Electronic Publication: 2015 Jun 29 (Print Publication: 2015).
DOI:	10.4137/CIN.S22941
Abstrakt:	Lung cancer remains the most prevalent malignancy and the primary cause of cancer-related deaths worldwide. Unique mutations patterns can be found in lung cancer subtypes, in individual cancers, or within a single tumor, and drugs that target these genetic mutations and signal transduction pathways are often beneficial to patients. In this study, we used the Ion Torrent AmpliSeq Cancer Panel to sequence 737 loci from 45 cancer-related genes and oncogenes to identify genetic mutations in 48 formalin-fixed, paraffin-embedded (FFPE) human lung cancer samples from Chinese patients. We found frequent mutations in EGFR, KRAS, PIK3CA, and TP53 genes. Moreover, we observed that a portion of the lung cancer samples harbored two or more mutations in these key genes. This study demonstrates the feasibility of using the Ion Torrent sequencing to efficiently identify genetic mutations in individual tumors for targeted lung cancer therapy.
Databáze:	MEDLINE
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