Naked-eye nanobiosensor for therapeutic drug monitoring of methotrexate.

Autor:	Yockell-Lelièvre H; Département de chimie, Université de Montréal, CP 6128 Succ. Centre-Ville, Montreal, QC, CanadaH3C 3J7. jf.masson@umontreal.ca., Bukar N; Département de chimie, Université de Montréal, CP 6128 Succ. Centre-Ville, Montreal, QC, CanadaH3C 3J7. jf.masson@umontreal.ca., Toulouse JL; Département de chimie, Université de Montréal, CP 6128 Succ. Centre-Ville, Montreal, QC, CanadaH3C 3J7. jf.masson@umontreal.ca., Pelletier JN; Département de chimie, Université de Montréal, CP 6128 Succ. Centre-Ville, Montreal, QC, CanadaH3C 3J7. jf.masson@umontreal.ca and PROTEO., Masson JF; Département de chimie, Université de Montréal, CP 6128 Succ. Centre-Ville, Montreal, QC, CanadaH3C 3J7. jf.masson@umontreal.ca and Centre for self-assembled chemical structures (CSACS).
Jazyk:	angličtina
Zdroj:	The Analyst [Analyst] 2016 Jan 21; Vol. 141 (2), pp. 697-703.
DOI:	10.1039/c5an00996k
Abstrakt:	Sensing of methotrexate at clinically-relevant concentrations was achieved with a plasmon-coupling assay. In this assay, free methotrexate and folic acid Au nanoparticles competed for human dihydrofolate reductase (hDHFR)-functionalized Au nanoparticles (Au NP). The hDHFR-functionalized Au NPs were immobilized on a small glass sensor inserted in a portable 4-channel LSPR reader. This allowed rapid (minutes) and sensitive (nanomolar range) measurement of methotrexate concentration by means of total internal reflection plasmonic spectroscopy. The large bathochromic shifts of the plasmon-coupling assay led to striking colour changes visible to the naked eye for methotrexate at clinically-relevant concentrations. The results demonstrate the potential for therapeutic drug monitoring of a widely used chemotherapy agent, as assessed with the naked eye.
Databáze:	MEDLINE
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