A Single Amino Acid Substitution in an ORANGE Protein Promotes Carotenoid Overaccumulation in Arabidopsis.

Autor:	Yuan H; Robert W. Holley Center for Agriculture and Health, U.S. Department of Agriculture-Agricultural Research Service (H.Y., X.Z., Y.Y., T.W.T., L.L.), Plant Breeding and Genetics Section, School of Integrative Plant Science (H.Y., K.O., T.E.S., X.Z., C.R., L.L.), Plant Biology Section, School of Integrative Plant Science (M.V.P.), and Boyce Thompson Institute for Plant Research (Z.F.), Cornell University, Ithaca, New York 14853;Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, People's Republic of China (Y.L., Q.X., X.D.);University of Freiburg, Faculty of Biology II, D79104 Freiburg, Germany (R.W.); andNewe Ya'ar Research Center, Agricultural Research Organization, Ramat Yishay 30095, Israel (N.C., A.S., N.K., J.B., Y.T.)., Owsiany K; Robert W. Holley Center for Agriculture and Health, U.S. Department of Agriculture-Agricultural Research Service (H.Y., X.Z., Y.Y., T.W.T., L.L.), Plant Breeding and Genetics Section, School of Integrative Plant Science (H.Y., K.O., T.E.S., X.Z., C.R., L.L.), Plant Biology Section, School of Integrative Plant Science (M.V.P.), and Boyce Thompson Institute for Plant Research (Z.F.), Cornell University, Ithaca, New York 14853;Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, People's Republic of China (Y.L., Q.X., X.D.);University of Freiburg, Faculty of Biology II, D79104 Freiburg, Germany (R.W.); andNewe Ya'ar Research Center, Agricultural Research Organization, Ramat Yishay 30095, Israel (N.C., A.S., N.K., J.B., Y.T.)., Sheeja TE; Robert W. Holley Center for Agriculture and Health, U.S. Department of Agriculture-Agricultural Research Service (H.Y., X.Z., Y.Y., T.W.T., L.L.), Plant Breeding and Genetics Section, School of Integrative Plant Science (H.Y., K.O., T.E.S., X.Z., C.R., L.L.), Plant Biology Section, School of Integrative Plant Science (M.V.P.), and Boyce Thompson Institute for Plant Research (Z.F.), Cornell University, Ithaca, New York 14853;Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, People's Republic of China (Y.L., Q.X., X.D.);University of Freiburg, Faculty of Biology II, D79104 Freiburg, Germany (R.W.); andNewe Ya'ar Research Center, Agricultural Research Organization, Ramat Yishay 30095, Israel (N.C., A.S., N.K., J.B., Y.T.)., Zhou X; Robert W. Holley Center for Agriculture and Health, U.S. Department of Agriculture-Agricultural Research Service (H.Y., X.Z., Y.Y., T.W.T., L.L.), Plant Breeding and Genetics Section, School of Integrative Plant Science (H.Y., K.O., T.E.S., X.Z., C.R., L.L.), Plant Biology Section, School of Integrative Plant Science (M.V.P.), and Boyce Thompson Institute for Plant Research (Z.F.), Cornell University, Ithaca, New York 14853;Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, People's Republic of China (Y.L., Q.X., X.D.);University of Freiburg, Faculty of Biology II, D79104 Freiburg, Germany (R.W.); andNewe Ya'ar Research Center, Agricultural Research Organization, Ramat Yishay 30095, Israel (N.C., A.S., N.K., J.B., Y.T.)., Rodriguez C; Robert W. Holley Center for Agriculture and Health, U.S. Department of Agriculture-Agricultural Research Service (H.Y., X.Z., Y.Y., T.W.T., L.L.), Plant Breeding and Genetics Section, School of Integrative Plant Science (H.Y., K.O., T.E.S., X.Z., C.R., L.L.), Plant Biology Section, School of Integrative Plant Science (M.V.P.), and Boyce Thompson Institute for Plant Research (Z.F.), Cornell University, Ithaca, New York 14853;Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, People's Republic of China (Y.L., Q.X., X.D.);University of Freiburg, Faculty of Biology II, D79104 Freiburg, Germany (R.W.); andNewe Ya'ar Research Center, Agricultural Research Organization, Ramat Yishay 30095, Israel (N.C., A.S., N.K., J.B., Y.T.)., Li Y; Robert W. Holley Center for Agriculture and Health, U.S. Department of Agriculture-Agricultural Research Service (H.Y., X.Z., Y.Y., T.W.T., L.L.), Plant Breeding and Genetics Section, School of Integrative Plant Science (H.Y., K.O., T.E.S., X.Z., C.R., L.L.), Plant Biology Section, School of Integrative Plant Science (M.V.P.), and Boyce Thompson Institute for Plant Research (Z.F.), Cornell University, Ithaca, New York 14853;Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, People's Republic of China (Y.L., Q.X., X.D.);University of Freiburg, Faculty of Biology II, D79104 Freiburg, Germany (R.W.); andNewe Ya'ar Research Center, Agricultural Research Organization, Ramat Yishay 30095, Israel (N.C., A.S., N.K., J.B., Y.T.)., Welsch R; Robert W. Holley Center for Agriculture and Health, U.S. Department of Agriculture-Agricultural Research Service (H.Y., X.Z., Y.Y., T.W.T., L.L.), Plant Breeding and Genetics Section, School of Integrative Plant Science (H.Y., K.O., T.E.S., X.Z., C.R., L.L.), Plant Biology Section, School of Integrative Plant Science (M.V.P.), and Boyce Thompson Institute for Plant Research (Z.F.), Cornell University, Ithaca, New York 14853;Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, People's Republic of China (Y.L., Q.X., X.D.);University of Freiburg, Faculty of Biology II, D79104 Freiburg, Germany (R.W.); andNewe Ya'ar Research Center, Agricultural Research Organization, Ramat Yishay 30095, Israel (N.C., A.S., N.K., J.B., Y.T.)., Chayut N; Robert W. Holley Center for Agriculture and Health, U.S. Department of Agriculture-Agricultural Research Service (H.Y., X.Z., Y.Y., T.W.T., L.L.), Plant Breeding and Genetics Section, School of Integrative Plant Science (H.Y., K.O., T.E.S., X.Z., C.R., L.L.), Plant Biology Section, School of Integrative Plant Science (M.V.P.), and Boyce Thompson Institute for Plant Research (Z.F.), Cornell University, Ithaca, New York 14853;Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, People's Republic of China (Y.L., Q.X., X.D.);University of Freiburg, Faculty of Biology II, D79104 Freiburg, Germany (R.W.); andNewe Ya'ar Research Center, Agricultural Research Organization, Ramat Yishay 30095, Israel (N.C., A.S., N.K., J.B., Y.T.)., Yang Y; Robert W. Holley Center for Agriculture and Health, U.S. Department of Agriculture-Agricultural Research Service (H.Y., X.Z., Y.Y., T.W.T., L.L.), Plant Breeding and Genetics Section, School of Integrative Plant Science (H.Y., K.O., T.E.S., X.Z., C.R., L.L.), Plant Biology Section, School of Integrative Plant Science (M.V.P.), and Boyce Thompson Institute for Plant Research (Z.F.), Cornell University, Ithaca, New York 14853;Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, People's Republic of China (Y.L., Q.X., X.D.);University of Freiburg, Faculty of Biology II, D79104 Freiburg, Germany (R.W.); andNewe Ya'ar Research Center, Agricultural Research Organization, Ramat Yishay 30095, Israel (N.C., A.S., N.K., J.B., Y.T.)., Thannhauser TW; Robert W. Holley Center for Agriculture and Health, U.S. Department of Agriculture-Agricultural Research Service (H.Y., X.Z., Y.Y., T.W.T., L.L.), Plant Breeding and Genetics Section, School of Integrative Plant Science (H.Y., K.O., T.E.S., X.Z., C.R., L.L.), Plant Biology Section, School of Integrative Plant Science (M.V.P.), and Boyce Thompson Institute for Plant Research (Z.F.), Cornell University, Ithaca, New York 14853;Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, People's Republic of China (Y.L., Q.X., X.D.);University of Freiburg, Faculty of Biology II, D79104 Freiburg, Germany (R.W.); andNewe Ya'ar Research Center, Agricultural Research Organization, Ramat Yishay 30095, Israel (N.C., A.S., N.K., J.B., Y.T.)., Parthasarathy MV; Robert W. Holley Center for Agriculture and Health, U.S. Department of Agriculture-Agricultural Research Service (H.Y., X.Z., Y.Y., T.W.T., L.L.), Plant Breeding and Genetics Section, School of Integrative Plant Science (H.Y., K.O., T.E.S., X.Z., C.R., L.L.), Plant Biology Section, School of Integrative Plant Science (M.V.P.), and Boyce Thompson Institute for Plant Research (Z.F.), Cornell University, Ithaca, New York 14853;Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, People's Republic of China (Y.L., Q.X., X.D.);University of Freiburg, Faculty of Biology II, D79104 Freiburg, Germany (R.W.); andNewe Ya'ar Research Center, Agricultural Research Organization, Ramat Yishay 30095, Israel (N.C., A.S., N.K., J.B., Y.T.)., Xu Q; Robert W. Holley Center for Agriculture and Health, U.S. Department of Agriculture-Agricultural Research Service (H.Y., X.Z., Y.Y., T.W.T., L.L.), Plant Breeding and Genetics Section, School of Integrative Plant Science (H.Y., K.O., T.E.S., X.Z., C.R., L.L.), Plant Biology Section, School of Integrative Plant Science (M.V.P.), and Boyce Thompson Institute for Plant Research (Z.F.), Cornell University, Ithaca, New York 14853;Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, People's Republic of China (Y.L., Q.X., X.D.);University of Freiburg, Faculty of Biology II, D79104 Freiburg, Germany (R.W.); andNewe Ya'ar Research Center, Agricultural Research Organization, Ramat Yishay 30095, Israel (N.C., A.S., N.K., J.B., Y.T.)., Deng X; Robert W. Holley Center for Agriculture and Health, U.S. Department of Agriculture-Agricultural Research Service (H.Y., X.Z., Y.Y., T.W.T., L.L.), Plant Breeding and Genetics Section, School of Integrative Plant Science (H.Y., K.O., T.E.S., X.Z., C.R., L.L.), Plant Biology Section, School of Integrative Plant Science (M.V.P.), and Boyce Thompson Institute for Plant Research (Z.F.), Cornell University, Ithaca, New York 14853;Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, People's Republic of China (Y.L., Q.X., X.D.);University of Freiburg, Faculty of Biology II, D79104 Freiburg, Germany (R.W.); andNewe Ya'ar Research Center, Agricultural Research Organization, Ramat Yishay 30095, Israel (N.C., A.S., N.K., J.B., Y.T.)., Fei Z; Robert W. Holley Center for Agriculture and Health, U.S. Department of Agriculture-Agricultural Research Service (H.Y., X.Z., Y.Y., T.W.T., L.L.), Plant Breeding and Genetics Section, School of Integrative Plant Science (H.Y., K.O., T.E.S., X.Z., C.R., L.L.), Plant Biology Section, School of Integrative Plant Science (M.V.P.), and Boyce Thompson Institute for Plant Research (Z.F.), Cornell University, Ithaca, New York 14853;Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, People's Republic of China (Y.L., Q.X., X.D.);University of Freiburg, Faculty of Biology II, D79104 Freiburg, Germany (R.W.); andNewe Ya'ar Research Center, Agricultural Research Organization, Ramat Yishay 30095, Israel (N.C., A.S., N.K., J.B., Y.T.)., Schaffer A; Robert W. Holley Center for Agriculture and Health, U.S. Department of Agriculture-Agricultural Research Service (H.Y., X.Z., Y.Y., T.W.T., L.L.), Plant Breeding and Genetics Section, School of Integrative Plant Science (H.Y., K.O., T.E.S., X.Z., C.R., L.L.), Plant Biology Section, School of Integrative Plant Science (M.V.P.), and Boyce Thompson Institute for Plant Research (Z.F.), Cornell University, Ithaca, New York 14853;Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, People's Republic of China (Y.L., Q.X., X.D.);University of Freiburg, Faculty of Biology II, D79104 Freiburg, Germany (R.W.); andNewe Ya'ar Research Center, Agricultural Research Organization, Ramat Yishay 30095, Israel (N.C., A.S., N.K., J.B., Y.T.)., Katzir N; Robert W. Holley Center for Agriculture and Health, U.S. Department of Agriculture-Agricultural Research Service (H.Y., X.Z., Y.Y., T.W.T., L.L.), Plant Breeding and Genetics Section, School of Integrative Plant Science (H.Y., K.O., T.E.S., X.Z., C.R., L.L.), Plant Biology Section, School of Integrative Plant Science (M.V.P.), and Boyce Thompson Institute for Plant Research (Z.F.), Cornell University, Ithaca, New York 14853;Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, People's Republic of China (Y.L., Q.X., X.D.);University of Freiburg, Faculty of Biology II, D79104 Freiburg, Germany (R.W.); andNewe Ya'ar Research Center, Agricultural Research Organization, Ramat Yishay 30095, Israel (N.C., A.S., N.K., J.B., Y.T.)., Burger J; Robert W. Holley Center for Agriculture and Health, U.S. Department of Agriculture-Agricultural Research Service (H.Y., X.Z., Y.Y., T.W.T., L.L.), Plant Breeding and Genetics Section, School of Integrative Plant Science (H.Y., K.O., T.E.S., X.Z., C.R., L.L.), Plant Biology Section, School of Integrative Plant Science (M.V.P.), and Boyce Thompson Institute for Plant Research (Z.F.), Cornell University, Ithaca, New York 14853;Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, People's Republic of China (Y.L., Q.X., X.D.);University of Freiburg, Faculty of Biology II, D79104 Freiburg, Germany (R.W.); andNewe Ya'ar Research Center, Agricultural Research Organization, Ramat Yishay 30095, Israel (N.C., A.S., N.K., J.B., Y.T.)., Tadmor Y; Robert W. Holley Center for Agriculture and Health, U.S. Department of Agriculture-Agricultural Research Service (H.Y., X.Z., Y.Y., T.W.T., L.L.), Plant Breeding and Genetics Section, School of Integrative Plant Science (H.Y., K.O., T.E.S., X.Z., C.R., L.L.), Plant Biology Section, School of Integrative Plant Science (M.V.P.), and Boyce Thompson Institute for Plant Research (Z.F.), Cornell University, Ithaca, New York 14853;Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, People's Republic of China (Y.L., Q.X., X.D.);University of Freiburg, Faculty of Biology II, D79104 Freiburg, Germany (R.W.); andNewe Ya'ar Research Center, Agricultural Research Organization, Ramat Yishay 30095, Israel (N.C., A.S., N.K., J.B., Y.T.)., Li L; Robert W. Holley Center for Agriculture and Health, U.S. Department of Agriculture-Agricultural Research Service (H.Y., X.Z., Y.Y., T.W.T., L.L.), Plant Breeding and Genetics Section, School of Integrative Plant Science (H.Y., K.O., T.E.S., X.Z., C.R., L.L.), Plant Biology Section, School of Integrative Plant Science (M.V.P.), and Boyce Thompson Institute for Plant Research (Z.F.), Cornell University, Ithaca, New York 14853;Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, People's Republic of China (Y.L., Q.X., X.D.);University of Freiburg, Faculty of Biology II, D79104 Freiburg, Germany (R.W.); andNewe Ya'ar Research Center, Agricultural Research Organization, Ramat Yishay 30095, Israel (N.C., A.S., N.K., J.B., Y.T.) ll37@cornell.edu.
Jazyk:	angličtina
Zdroj:	Plant physiology [Plant Physiol] 2015 Sep; Vol. 169 (1), pp. 421-31. Date of Electronic Publication: 2015 Jul 29.
DOI:	10.1104/pp.15.00971
Abstrakt:	Carotenoids are crucial for plant growth and human health. The finding of ORANGE (OR) protein as a pivotal regulator of carotenogenesis offers a unique opportunity to comprehensively understand the regulatory mechanisms of carotenoid accumulation and develop crops with enhanced nutritional quality. Here, we demonstrated that alteration of a single amino acid in a wild-type OR greatly enhanced its ability to promote carotenoid accumulation. Whereas overexpression of OR from Arabidopsis (Arabidopsis thaliana; AtOR) or from the agronomically important crop sorghum (Sorghum bicolor; SbOR) increased carotenoid levels up to 2-fold, expression of AtOR(His) (R90H) or SbOR(His) (R104H) variants dramatically enhanced carotenoid accumulation by up to 7-fold in the Arabidopsis calli. Moreover, we found that AtOR(Ala) (R90A) functioned similarly to AtOR(His) to promote carotenoid overproduction. Neither AtOR nor AtOR(His) greatly affected carotenogenic gene expression. AtOR(His) exhibited similar interactions with phytoene synthase (PSY) as AtOR in posttranscriptionally regulating PSY protein abundance. AtOR(His) triggered biogenesis of membranous chromoplasts in the Arabidopsis calli, which shared structures similar to chromoplasts found in the curd of the orange cauliflower (Brassica oleracea) mutant. By contrast, AtOR did not cause plastid-type changes in comparison with the controls, but produced plastids containing larger and electron-dense plastoglobuli. The unique ability of AtOR(His) in mediating chromoplast biogenesis is responsible for its induced carotenoid overproduction. Our study demonstrates OR(His/Ala) as powerful tools for carotenoid enrichment in plants, and provides insights into the mechanisms underlying OR(His)-regulated carotenoid accumulation. (© 2015 American Society of Plant Biologists. All Rights Reserved.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu