Allele and haplotype frequencies of HLA-A, B, C, DRB1 and DQB1 genes in polytransfused patients in ethnically diverse populations from Brazil.

Autor: Rodrigues C; Sciences Basic Health Department, Laboratory of Immunogenetics, Maringa State University, Maringa, PR, Brazil., Macedo LC; Sciences Basic Health Department, Laboratory of Immunogenetics, Maringa State University, Maringa, PR, Brazil., Bruder AV; Sciences Basic Health Department, Laboratory of Immunogenetics, Maringa State University, Maringa, PR, Brazil., Quintero Fd; Sciences Basic Health Department, Laboratory of Immunogenetics, Maringa State University, Maringa, PR, Brazil., de Alencar JB; Sciences Basic Health Department, Laboratory of Immunogenetics, Maringa State University, Maringa, PR, Brazil., Sell AM; Sciences Basic Health Department, Laboratory of Immunogenetics, Maringa State University, Maringa, PR, Brazil., Visentainer JE; Sciences Basic Health Department, Laboratory of Immunogenetics, Maringa State University, Maringa, PR, Brazil.
Jazyk: angličtina
Zdroj: International journal of immunogenetics [Int J Immunogenet] 2015 Oct; Vol. 42 (5), pp. 322-8. Date of Electronic Publication: 2015 Jul 30.
DOI: 10.1111/iji.12206
Abstrakt: The red blood transfusion is a practice often used in patients with haematological and oncological diseases. However, the investigation of human leucocyte antigen (HLA) system frequency in these individuals is of great importance because multiple transfusions may lead to HLA alloimmunization. Brazil is a country that was colonized by many other ethnicities, leading to a mixed ethnicity and regionalized population. In view of the importance of HLA typing in these patients, the aim of this study was to investigate the allele and haplotype frequencies from polytransfused patients from three different regions from Brazil. HLA-A, HLA-B, HLA-C, HLA-DRB1 and HLA-DQB1 genotyping of 366 patients was performed by PCR-SSO, based on the Luminex technology (One Lambda(®) ), and the anti-HLA class I and class II antibodies were analysed using LabScreen Single Antigen Antibody Detection (One Lambda, Inc.). Allele and haplotype frequencies of polytransfused patients of three regions from Brazil were obtained using the Arlequin program. The most frequent allele frequencies observed were HLA-A*02, A*03, B*15, B*35, B*51, C*07, C*04, C*03, DRB1*13, DRB1*11, DRB1*07, DRB1*03, DRB1*01, DQB1*03, DQB1*02, DQB1*06 and DQB1*05. There were differences between the groups for allele variants HLA-B*57 (between Group 1 and Group 2) and HLA-C*12 (between Group 1 and Group 3). The most frequent haplotypes found in the sample were HLA-A*01B*08DRB1*03, DRBI*07DQB1*02, DRB1*01DQB1*05, DRB1*13DQB1*06 and A*02B*35. HLA class I and II antibodies were detected in 77.9% and 63.9% patients, respectively, while the both alloantibodies were detected in 62 (50.9%) patients. In conclusion, the HLA typing for polytransfused patients in each region has a great importance, as seen in this study; individuals from different regions from Brazil have HLA distribution not completely homogeneous.
(© 2015 John Wiley & Sons Ltd.)
Databáze: MEDLINE
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