Experimental infection by Trypanosoma evansi in sheep: Occurrence of transplacental transmission and mice infection by parasite present in the colostrum and milk of infected ewes.

Autor: Campigotto G; Department of Animal Science, Universidade do Estado de Santa Catarina - UDESC, Chapecó, SC, Brazil., Da Silva AS; Department of Animal Science, Universidade do Estado de Santa Catarina - UDESC, Chapecó, SC, Brazil. Electronic address: aleksandro_ss@yahoo.com.br., Volpato A; Department of Animal Science, Universidade do Estado de Santa Catarina - UDESC, Chapecó, SC, Brazil., Balzan A; Department of Animal Science, Universidade do Estado de Santa Catarina - UDESC, Chapecó, SC, Brazil., Radavelli WM; Department of Animal Science, Universidade do Estado de Santa Catarina - UDESC, Chapecó, SC, Brazil., Soldá NM; Department of Animal Science, Universidade do Estado de Santa Catarina - UDESC, Chapecó, SC, Brazil., Grosskopf HM; Department of Animal Science, Universidade do Estado de Santa Catarina - UDESC, Chapecó, SC, Brazil., Stefani LM; Department of Animal Science, Universidade do Estado de Santa Catarina - UDESC, Chapecó, SC, Brazil., Bianchi AE; Department of Animal Science, Universidade Federal do Paraná - UFPR, Curitiba, PR, Brazil., Monteiro SG; Department of Microbiology and Parasitology, Universidade Federal de Santa Maria - UFSM, Santa Maria, RS, Brazil., Tonin AA; Department of Microbiology and Parasitology, Universidade Federal de Santa Maria - UFSM, Santa Maria, RS, Brazil., Weiss PH; Department of Veterinary Medicine, UDESC, Lages, SC, Brazil., Miletti LC; Department of Veterinary Medicine, UDESC, Lages, SC, Brazil., Lopes ST; Department of Large Animal Medicine, UFSM, Santa Maria, RS, Brazil.
Jazyk: angličtina
Zdroj: Veterinary parasitology [Vet Parasitol] 2015 Sep 15; Vol. 212 (3-4), pp. 123-9. Date of Electronic Publication: 2015 Jul 09.
DOI: 10.1016/j.vetpar.2015.07.007
Abstrakt: The aims of this study were to evaluate vertical transmission of Trypanosoma evansi in sheep experimentally infected, in addition to the mammary transmission by colostrum or milk of these infected sheep to mice. Three pregnant sheep were used: one uninfected, four months pregnant (Sheep A); and two (Sheep B and C) infected intravenously by T. evansi trypomastigotes (4.6×10(6) per animal) on the third (Sheep C) and fourth (Sheep B) month of pregnancy. Both infected sheep developed low and oscillating parasitemia measured by blood smears. Hemogram was performed at seven day intervals, showing anemia, leukocytosis, and lymphocytosis on sheep B and C. Three sheep had twins, where sheep A delivered healthy lambs and both infected sheep had delivered at least one stillborn. Additionally, lambs from sheep B and C died 24 and 72 h post-partum, respectively. Before colostrum intake, four lambs from infected sheep were positives for T. evansi according to blood smear evaluation, serology (CATT/T. evansi), and PCR. Sheep colostrum and milk samples collected from the first four days post-partum were positives for T. evansi on PCR, and these samples were able to infect seven mice (out of 10) orally (n=4/5) and intraperitoneally (n=3/5). Therefore, we conclude that the vertical transmission of T. evansi occurs in pregnant sheep, in addition to a strong possibility of the transmission by colostrum and milk.
(Copyright © 2015 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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