Predictors for MTB Culture-Positivity among HIV-Infected Smear-Negative Presumptive Tuberculosis Patients in Uganda: Application of New Tuberculosis Diagnostic Technology.

Autor: Nakiyingi L; Infectious Diseases Institute, Makerere University College of Health Sciences, Kampala, Uganda; Department of Medicine, School of Medicine, Makerere University College of Heath Sciences, Kampala, Uganda., Nonyane BA; Department of International Health, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland, United States of America., Ssengooba W; Department of Medical Microbiology, Makerere University College of Heath Sciences, Kampala, Uganda., Kirenga BJ; Department of Medicine, School of Medicine, Makerere University College of Heath Sciences, Kampala, Uganda., Nakanjako D; Infectious Diseases Institute, Makerere University College of Health Sciences, Kampala, Uganda; Department of Medicine, School of Medicine, Makerere University College of Heath Sciences, Kampala, Uganda., Lubega G; Infectious Diseases Institute, Makerere University College of Health Sciences, Kampala, Uganda., Byakika-Kibwika P; Infectious Diseases Institute, Makerere University College of Health Sciences, Kampala, Uganda; Department of Medicine, School of Medicine, Makerere University College of Heath Sciences, Kampala, Uganda., Joloba ML; Department of Medical Microbiology, Makerere University College of Heath Sciences, Kampala, Uganda., Ellner JJ; Boston Medical Center, Boston University School of Medicine, Boston, MA, United States of America., Dorman SE; Division of Infectious Diseases, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America., Mayanja-Kizza H; Department of Medicine, School of Medicine, Makerere University College of Heath Sciences, Kampala, Uganda., Manabe YC; Infectious Diseases Institute, Makerere University College of Health Sciences, Kampala, Uganda; Division of Infectious Diseases, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2015 Jul 29; Vol. 10 (7), pp. e0133756. Date of Electronic Publication: 2015 Jul 29 (Print Publication: 2015).
DOI: 10.1371/journal.pone.0133756
Abstrakt: Background: The existing World Health Organization diagnostic algorithms for smear-negative TB perform poorly in HIV-infected individuals. New TB diagnostics such as urine TB lipoarabinomannan (LAM) could improve the accuracy and reduce delays in TB diagnosis in HIV-infected smear-negative presumptive TB. We sought to determine predictors for MTB culture-positivity among these patients.
Methods: This study was nested into a prospective evaluation of HIV-infected outpatients and inpatients clinically suspected to have TB who were screened by smear-microscopy on two spot sputum samples. Data on socio-demographics, clinical symptoms, antiretroviral therapy, CXR, CD4 count, mycobacterial sputum and blood cultures and TB-LAM were collected. Logistic regression and conditional inference tree analysis were used to determine the most predictive indicators for MTB culture-positivity.
Results: Of the 418 smear-negative participants [female, 64%; median age (IQR) 32 (28-39) years, median CD4 106 (IQR 22 - 298) cells/mm3], 96/418 (23%) were sputum and/ or blood culture-positive for Mycobacterium tuberculosis (MTB) complex. Abnormal CXR (aOR 3.68, 95% CI 1.76- 7.71, p=0.001) and positive urine TB-LAM (aOR 6.21, 95% CI 3.14-12.27, p< 0.001) were significantly associated with MTB culture-positivity. Previous TB treatment (aOR 0.41, 95% CI 0.17-0.99, p=0.049) reduced the likelihood of a positive MTB culture. A conditional inference tree analysis showed that positive urine TB-LAM and abnormal CXR were the most predictive indicators of MTB culture-positivity. A combination of urine TB-LAM test and CXR had sensitivity and specificity of 50% and 86.1% respectively overall, and 70.8% and 84.1% respectively among those with CD4<100 cells/mm3.
Conclusions: A positive urine TB-LAM test and an abnormal CXR significantly predict MTB culture-positivity among smear-negative HIV-infected presumptive TB patients while previous TB treatment reduces the likelihood of a positive MTB culture. Validation studies to assess the performance of diagnostic algorithms that include urine TB-LAM in the diagnosis of smear-negative TB in HIV-infected individuals are warranted.
Databáze: MEDLINE
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