A high-throughput screening assay for distinguishing nitrile hydratases from nitrilases.
| Autor: | Angelini LM; Instituto de of Biociências, Universidade Estadual Paulista 'Julio de Mesquita Filho', Rio Claro, SP, Brazil., da Silva AR; Instituto de of Biociências, Universidade Estadual Paulista 'Julio de Mesquita Filho', Rio Claro, SP, Brazil., Rocco Lde F; Instituto de of Biociências, Universidade Estadual Paulista 'Julio de Mesquita Filho', Rio Claro, SP, Brazil., Milagre CD; Departamento de Química Orgânia, Instituto de Química, Universidade Estadual Paulista 'Julio de Mesquita Filho', Araraquara, SP, Brazil. |
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| Jazyk: | angličtina |
| Zdroj: | Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] [Braz J Microbiol] 2015 Mar 31; Vol. 46 (1), pp. 113-6. Date of Electronic Publication: 2015 Mar 31 (Print Publication: 2015). |
| DOI: | 10.1590/S1517-838246120130851 |
| Abstrakt: | A modified colorimetric high-throughput screen based on pH changes combined with an amidase inhibitor capable of distinguishing between nitrilases and nitrile hydratases. This enzymatic screening is based on a binary response and is suitable for the first step of hierarchical screening projects. |
| Databáze: | MEDLINE |
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