[Atomic force microscopy fishing of gp120 on immobilized aptamer and its mass spectrometry identification].

Autor:	Bukharina NS; Institute of Biomedical Chemistry, Moscow, Russia., Ivanov YD; Institute of Biomedical Chemistry, Moscow, Russia., Pleshakova TO; Institute of Biomedical Chemistry, Moscow, Russia., Frantsuzov PA; Institute of Biomedical Chemistry, Moscow, Russia., Andreeva EY; Institute of Biomedical Chemistry, Moscow, Russia., Kaysheva AL; Institute of Biomedical Chemistry, Moscow, Russia., Izotov AA; Institute of Biomedical Chemistry, Moscow, Russia., Pavlova TI; Institute of Biomedical Chemistry, Moscow, Russia., Ziborov VS; Institute of Biomedical Chemistry, Moscow, Russia., Radko SP; Institute of Biomedical Chemistry, Moscow, Russia., Archakov AI; Institute of Biomedical Chemistry, Moscow, Russia.
Jazyk:	ruština
Zdroj:	Biomeditsinskaia khimiia [Biomed Khim] 2015 May-Jun; Vol. 61 (3), pp. 363-72.
DOI:	10.18097/PBMC20156103363
Abstrakt:	A method of atomic force microscopy-based fishing (AFM fishing) has been developed for protein detection in the analyte solution using a chip with an immobilized aptamer. This method is based on the biospecific fishing of a target protein from a bulk solution onto the small AFM chip area with the immobilized aptamer to this protein used as the molecular probe. Such aptamer-based approach allows to increase an AFM image contrast compared to the antibody-based approach. Mass spectrometry analysis used after the biospecific fishing to identify the target protein on the AFM chip has proved complex formation. Use of the AFM chip with the immobilized aptamer avoids interference of the antibody and target protein peaks in a mass spectrum.
Databáze:	MEDLINE
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