Accumulation of amino-polyvinyl alcohol-coated superparamagnetic iron oxide nanoparticles in bone marrow: implications for local stromal cells.

Autor:	Schulze F; Julius Wolff Institute, Charité - Universitätsmedizin Berlin, 13353 Berlin, Germany.; Berlin-Brandenburg School for Regenerative Therapies, Charité - Universitätsmedizin Berlin, 13353 Berlin, Germany., Gramoun A; Department of Radiology, Geneva University Hospitals & University of Geneva, 1205 Geneva, Switzerland.; Department of Oral Biology, Faculty of Oral & Dental Medicine, Cairo University, Cairo, Egypt., Crowe LA; Department of Radiology, Geneva University Hospitals & University of Geneva, 1205 Geneva, Switzerland., Dienelt A; Julius Wolff Institute, Charité - Universitätsmedizin Berlin, 13353 Berlin, Germany.; Berlin-Brandenburg Center for Regenerative Therapies, Charité - Universitätsmedizin Berlin, 13353 Berlin, Germany., Akcan T; Julius Wolff Institute, Charité - Universitätsmedizin Berlin, 13353 Berlin, Germany., Hofmann H; Laboratory of Powder Technology, Ecole Polytechnique Fédérale de Lausanne (EPFL), 1015 Lausanne, Switzerland., Vallée JP; Department of Radiology, Geneva University Hospitals & University of Geneva, 1205 Geneva, Switzerland., Duda GN; Julius Wolff Institute, Charité - Universitätsmedizin Berlin, 13353 Berlin, Germany.; Berlin-Brandenburg Center for Regenerative Therapies, Charité - Universitätsmedizin Berlin, 13353 Berlin, Germany., Ode A; Julius Wolff Institute, Charité - Universitätsmedizin Berlin, 13353 Berlin, Germany.; Berlin-Brandenburg Center for Regenerative Therapies, Charité - Universitätsmedizin Berlin, 13353 Berlin, Germany.
Jazyk:	angličtina
Zdroj:	Nanomedicine (London, England) [Nanomedicine (Lond)] 2015 Jul; Vol. 10 (14), pp. 2139-51.
DOI:	10.2217/nnm.15.62
Abstrakt:	Aims: First, it will be investigated if amino-polyvinyl alcohol-coated superparamagnetic iron oxide nanoparticles (A-PVA-SPIONs) are suitable for MRI contrast enhancement in bone marrow. Second, the impact of A-PVA-SPION exposure in vivo on the viability and key functions of local bone marrow stromal cells (BMSCs) will be investigated. Material & Methods: Animals were systemically injected with A-PVA-SPIONs, followed by a 7-day survival time. Accumulation of A-PVA-SPIONs was confirmed by MRI, histology and inductively coupled plasma optical emission spectrometry. BMSCs were isolated from bone marrow for in vitro assessment of their viability and regenerative key functions. Results: In this study, A-PVA-SPIONs were found to accumulate in bone marrow and increase the BMSCs' metabolic activity and migration rate. Conclusion: A-PVA-SPIONs appear suitable for contrast enhancement in bone marrow while our data suggest an influence on the BMSCs biology that necessitates future research.
Databáze:	MEDLINE
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