| Autor: |
Carnevale S, Pantano ML, Kamenetzky L, Malandrini JB, Soria CC, Velásquez JN |
| Jazyk: |
angličtina |
| Zdroj: |
Acta parasitologica [Acta Parasitol] 2015 Jun; Vol. 60 (2), pp. 211-7. |
| DOI: |
10.1515/ap-2015-0030 |
| Abstrakt: |
Fasciolosis is an important parasitic zoonosis considered the most important helminth infection of ruminants in tropical countries. The aim of this study was to develop a PCR assay for the sensitive and specific detection of F. hepatica in formalin preserved sheep faeces. A 405-bp fragment of the cytochrome c oxidase subunit 1 gene of F. hepatica was amplified from stool samples of infected sheep. The PCR assay showed a detection limit of 20 pg of F. hepatica DNA. No cross-reactions were observed with samples containing coccidian oocysts or gastrointestinal nematodes eggs. Our PCR technique showed to be effective for specific detection of F. hepatica infections in sheep. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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