Tyrosinase conjugated reduced graphene oxide based biointerface for bisphenol A sensor.

Autor: Reza KK; Biomedical Instrumentation Section, National Physical Laboratory (CSIR), Dr. K.S. Krishnan Marg, New Delhi 110012, India. Electronic address: kamilreza@gmail.com., Ali MA; Biomedical Instrumentation Section, National Physical Laboratory (CSIR), Dr. K.S. Krishnan Marg, New Delhi 110012, India., Srivastava S; Biomedical Instrumentation Section, National Physical Laboratory (CSIR), Dr. K.S. Krishnan Marg, New Delhi 110012, India., Agrawal VV; Biomedical Instrumentation Section, National Physical Laboratory (CSIR), Dr. K.S. Krishnan Marg, New Delhi 110012, India., Biradar AM; Biomedical Instrumentation Section, National Physical Laboratory (CSIR), Dr. K.S. Krishnan Marg, New Delhi 110012, India.
Jazyk: angličtina
Zdroj: Biosensors & bioelectronics [Biosens Bioelectron] 2015 Dec 15; Vol. 74, pp. 644-51. Date of Electronic Publication: 2015 Jul 11.
DOI: 10.1016/j.bios.2015.07.020
Abstrakt: We have fabricated a nanocomposite of reduced graphene oxide (rGO) sheets and chitosan (Cn) polymer based highly sensitive electrochemical biosensor for detection of bisphenol A (BPA). The two-dimensional structure and chemical functionality of rGO and Cn provide an excellent electrode surface for loading of tyrosinase enzyme molecules. This rGO-Cn nanocomposite is capable of effectively utilizing their superior conductivity, larger effective surface area and superior electrochemical performance due to its synergistic effect between rGO and Cn. The structural, morphological and electrochemical characterizations of nanocomposite sheets have been performed by electron microscopy, X-ray diffraction, FTIR and Potentiostat/Galvanostat techniques. This fabricated biosensor is sensitive to nanomolar (0.74 nM) concentration of BPA and detection time is 10s compared to conventional BPA ELISA kit (0.3 µg/L and 2.5h). The rGO-Cn based biosensor exhibits a higher sensitivity (83.3 µA nM(-1) cm(-2)), wider linearity (0.01-50 µM) with good selectivity towards BPA. This biosensor is capable to quantify real sample of BPA using packaged drinking water bottles. This rGO-Cn nanocomposite sheets emerges as a potential electrode material for detection of other estrogenic substrate.
(Copyright © 2015 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: