| Autor: |
Sousa L; Laboratório de Bioquímica Celular, Universidade Federal de São João del Rei, Campus Centro-Oeste Dona Lindú, Av Sebastião Gonçalves Coelho 400, 35501-296, Divinópolis, Brazil., Garcia IJ; Laboratório de Bioquímica Celular, Universidade Federal de São João del Rei, Campus Centro-Oeste Dona Lindú, Av Sebastião Gonçalves Coelho 400, 35501-296, Divinópolis, Brazil., Costa TG; Laboratório de Bioquímica Celular, Universidade Federal de São João del Rei, Campus Centro-Oeste Dona Lindú, Av Sebastião Gonçalves Coelho 400, 35501-296, Divinópolis, Brazil., Silva LN; Laboratório de Bioquímica Celular, Universidade Federal de São João del Rei, Campus Centro-Oeste Dona Lindú, Av Sebastião Gonçalves Coelho 400, 35501-296, Divinópolis, Brazil., Renó CO; Laboratório de Bioquímica Celular, Universidade Federal de São João del Rei, Campus Centro-Oeste Dona Lindú, Av Sebastião Gonçalves Coelho 400, 35501-296, Divinópolis, Brazil., Oliveira ES; Laboratório de Biologia Molecular, Universidade Federal de São João del Rei, Campus Centro-Oeste Dona Lindú, Av Sebastião Gonçalves Coelho 400, 35501-296, Divinópolis, Brazil., Tilelli CQ; Laboratório de Estudos em Neurociências das Epilepsias e Comorbidades, Universidade Federal de São João del Rei, Campus Centro-Oeste Dona Lindú, Av Sebastião Gonçalves Coelho 400, 35501-296, Divinópolis, Brazil., Santos LL; Laboratório de Biologia Molecular, Universidade Federal de São João del Rei, Campus Centro-Oeste Dona Lindú, Av Sebastião Gonçalves Coelho 400, 35501-296, Divinópolis, Brazil., Cortes VF; Laboratório de Bioquímica Celular, Universidade Federal de São João del Rei, Campus Centro-Oeste Dona Lindú, Av Sebastião Gonçalves Coelho 400, 35501-296, Divinópolis, Brazil., Santos HL; Laboratório de Bioquímica Celular, Universidade Federal de São João del Rei, Campus Centro-Oeste Dona Lindú, Av Sebastião Gonçalves Coelho 400, 35501-296, Divinópolis, Brazil., Barbosa LA; Laboratório de Bioquímica Celular, Universidade Federal de São João del Rei, Campus Centro-Oeste Dona Lindú, Av Sebastião Gonçalves Coelho 400, 35501-296, Divinópolis, Brazil. |
| Abstrakt: |
Iron is an essential chemical element for human life. However, in some pathological conditions, such as hereditary hemochromatosis type 1 (HH1), iron overload induces the production of reactive oxygen species that may lead to lipid peroxidation and a change in the plasma-membrane lipid profile. In this study, we investigated whether iron overload interferes with the Na,K-ATPase activity of the plasma membrane by studying erythrocytes that were obtained from the whole blood of patients suffering from iron overload. Additionally, we treated erythrocytes of normal subjects with 0.8 mM H2O2 and 1 μM FeCl3 for 24 h. We then analyzed the lipid profile, lipid peroxidation and Na,K-ATPase activity of plasma membranes derived from these cells. Iron overload was more frequent in men (87.5%) than in women and was associated with an increase (446%) in lipid peroxidation, as indicated by the amount of the thiobarbituric acid reactive substances (TBARS) and an increase (327%) in the Na,K-ATPase activity in the plasma membrane of erythrocytes. Erythrocytes treated with 1 μM FeCl3 for 24 h showed an increase (132%) in the Na,K-ATPase activity but no change in the TBARS levels. Iron treatment also decreased the cholesterol and phospholipid content of the erythrocyte membranes and similar decreases were observed in iron overload patients. In contrast, erythrocytes treated with 0.8 mM H2O2 for 24 h showed no change in the measured parameters. These results indicate that erythrocytes from patients with iron overload exhibit higher Na,K-ATPase activity compared with normal subjects and that this effect is specifically associated with altered iron levels. |
