| Autor: |
Crocomo LF; Rua dos Girassóis,n.278 - Bairro Convívio - CEP: 18605-240 Botucatu - S.P.,Brazil., Marques Filho WC; Department of Animal Reproduction and Veterinary Radiology - School of Veterinary Medicine and Animal Science (FMVZ),São Paulo State University (UNESP) - Botucatu,Brazil., Ackermann CL; Department of Animal Reproduction and Veterinary Radiology - School of Veterinary Medicine and Animal Science (FMVZ),São Paulo State University (UNESP) - Botucatu,Brazil., Paschoal DM; Department of Animal Reproduction and Veterinary Radiology - School of Veterinary Medicine and Animal Science (FMVZ),São Paulo State University (UNESP) - Botucatu,Brazil., Guastali MD; Department of Animal Reproduction and Veterinary Radiology - School of Veterinary Medicine and Animal Science (FMVZ),São Paulo State University (UNESP) - Botucatu,Brazil., Dias Maziero RR; Department of Animal Reproduction and Veterinary Radiology - School of Veterinary Medicine and Animal Science (FMVZ),São Paulo State University (UNESP) - Botucatu,Brazil., Sudano MJ; Laboratory of Genetics and Animal Breeding,Federal University of Pampa,Uruguaiana,RS,Brazil., Landim-Alvarenga Fda C; Department of Animal Reproduction and Veterinary Radiology - School of Veterinary Medicine and Animal Science (FMVZ),São Paulo State University (UNESP) - Botucatu,Brazil., Bicudo SD; Department of Animal Reproduction and Veterinary Radiology - School of Veterinary Medicine and Animal Science (FMVZ),São Paulo State University (UNESP) - Botucatu,Brazil. |
| Jazyk: |
angličtina |
| Zdroj: |
Zygote (Cambridge, England) [Zygote] 2016 Apr; Vol. 24 (2), pp. 310-8. Date of Electronic Publication: 2015 Jul 14. |
| DOI: |
10.1017/S0967199415000234 |
| Abstrakt: |
Temporary meiosis arrest with cyclin-dependent kinases inhibitors has been proposed in order to improve the quality of in vitro matured oocytes. In sheep, however, this phenomenon has been rarely investigated. Therefore, the present study aimed to evaluate the effect of different incubation times with roscovitine on nuclear maturation and cumulus cell expansion of sheep cumulus-oocyte complexes (COCs). For this, COCs were cultured for 0, 6, 12 or 20 h in basic maturation medium (Control) containing 75 μM roscovitine (Rosco). After, they were in vitro matured (IVM) for 18 h in the presence of luteinizing hormone (LH) and follicle-stimulating hormone (FSH). At the end of each treatment, cumulus cell expansion and nuclear maturation were assessed under a stereomicroscope and by Hoechst 33342 staining, respectively. In the Control and Rosco groups, the absence of cumulus cell expansion prevailed at 0, 6, 12 and 20 h. After IVM for 18 h, total cumulus cell expansion in the Rosco treatments was dependent on the exposure time to roscovitine. A significantly high percentage of oocytes treated with roscovitine for 6 h (87%), 12 h or 20 h (65%) were arrested at the germinal vesicle (GV) stage. In contrast, 23% GVBD, 54% metaphase I (MI) and 61% MII oocytes were observed in the Control groups at 6, 12 and 20 h, respectively. In all treatments, a significant percentage of oocytes reached MII after IVM for 18 h. Therefore, roscovitine reversibly arrested the meiosis of sheep oocytes during different culture times with the maximal efficiency of meiotic inhibition reached at 6 h. In addition, reversibility of its inhibitory action on cumulus cells was exposure-time dependent. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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