Absorbable bioprosthesis for the treatment of bile duct injury in an experimental model.

Autor: Montalvo-Javé EE; Departamento de Cirugía, Facultad de Medicina, UNAM, Mexico; Servicio de Gastroenterología, Fundación Clínica, Médica Sur, Mexico; Servicio de Cirugía General, Hospital General de México, Mexico. Electronic address: montalvoeduardo@hotmail.com., Mendoza Barrera GE; Departamento de Cirugía, Facultad de Medicina, UNAM, Mexico., Valderrama Treviño AI; Departamento de Cirugía, Facultad de Medicina, UNAM, Mexico., Piña Barba MC; Laboratorio de Biomateriales, Instituto de Investigación de Materiales, UNAM, Mexico., Montalvo-Arenas C; Departamento de Biología Celular y Tisular, Facultad de Medicina, UNAM, Mexico., Rojas Mendoza F; Servicio de Gastroenterología, Fundación Clínica, Médica Sur, Mexico., León Mancilla B; Departamento de Cirugía, Facultad de Medicina, UNAM, Mexico., García Pineda MA; Departamento de Cirugía, Facultad de Medicina, UNAM, Mexico., Jaime Limón Á; Departamento de Cirugía, Facultad de Medicina, UNAM, Mexico., Albores Saavedra J; Servicio de Patología, Fundación Clínica Médica Sur, Mexico., Tapia-Jurado J; Departamento de Cirugía, Facultad de Medicina, UNAM, Mexico.
Jazyk: angličtina
Zdroj: International journal of surgery (London, England) [Int J Surg] 2015 Aug; Vol. 20, pp. 163-9. Date of Electronic Publication: 2015 Jul 09.
DOI: 10.1016/j.ijsu.2015.06.074
Abstrakt: Introduction: Cholecystectomy is a common surgical procedure in which complications may occur, such as injury to the biliary tract, which are associated with high morbidity. The aim of this study was to demonstrate the efficacy of a polymer-based absorbable bioprosthesis with bone scaffold for the treatment of bile duct injury in an animal model.
Materials and Methods: An absorbable bioprosthesis was used to replace the common bile duct in 15 pigs which were divided into 3 groups with different follow-ups at 1, 3 and 6 months. The animals were anesthetized at these time points and laboratory tests, Magnetic Resonance Cholangiopancreatogram [MRCP], Choledochoscopy using Spyglass and Endoscopic retrograde Cholangiopancreatogram [ERCP] were performed. After radiological evaluation was complete, the animals were euthanized and histological and immunohistochemical analyses were performed.
Results: Liver function tests at different time points demonstrated no significant changes. No mortality or postoperative complications were found in any of the experimental models. Imaging studies ([MRCP], [ERCP] and Choledochoscopy with SpyGlass(™)) showed absence of stenosis or obstruction in all the experimental models.
Discussion: Histological and immunohistochemical staining (CK19 and MUC5+) revealed the presence of biliary epithelium with intramural biliary glands in all the experimental models. There was no stenosis or obstruction in the bile duct.
Conclusions: The bioprosthesis served as scaffolding for tissue regeneration. There was no postoperative complication at 6 months follow-up. This bioprosthesis could be used to replace the bile duct in cancer or bile duct injury. The bioprosthesis may allow different modeling depending on the type of bile duct injury.
(Copyright © 2015 IJS Publishing Group Limited. Published by Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: