| Autor: |
Kostryukova TS, Ivanovskaya NP, Zatonsky GV, Osin NS, Vasilev NV |
| Jazyk: |
ruština |
| Zdroj: |
Bioorganicheskaia khimiia [Bioorg Khim] 2015 Mar-Apr; Vol. 41 (2), pp. 212-7. |
| DOI: |
10.1134/s1068162015010094 |
| Abstrakt: |
In order to develop new markers for immunofluorescence time-resolved luminescence analysis tetraketodiester carbazole series was obtained. Compound contains methoxycarbonyl reactive groups, separated from the 1,3-dicarbonyl chelating fragment by difluoromethylene spacers (CF2)4. Complexes of this compound with the ions Eu3+ are stable in aqueous solution and possess a long-living intensive luminescence with the main peaks: emission--in the area of 615 nm and excitation--in the 380-390 nm, that distinguishes them from most used analogues. Conjugate of streptavidin to compound was obtained and its fluorescent spectral characteristics allow to use it as a universal reagent for various schemes of biological microanalysis. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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