| Autor: |
Parlapally S; a Centre for Plant Molecular Biology , Osmania University , Hyderabad 500 007 , India.; b Department of Pharmacy and Biotechnology , University College of Technology, Osmania University , Hyderabad 500 007 , India., Cherukupalli N; a Centre for Plant Molecular Biology , Osmania University , Hyderabad 500 007 , India., Bhumireddy SR; c National Centre for Mass Spectrometry , CSIR-Indian Institute of Chemical Technology , Hyderabad 500 007 Telangana , India.; d Academy of Scientific and Innovative Research, CSIR-IICT , Hyderabad , India., Sripadi P; c National Centre for Mass Spectrometry , CSIR-Indian Institute of Chemical Technology , Hyderabad 500 007 Telangana , India.; d Academy of Scientific and Innovative Research, CSIR-IICT , Hyderabad , India., Anisetti R; b Department of Pharmacy and Biotechnology , University College of Technology, Osmania University , Hyderabad 500 007 , India., Giri CC; a Centre for Plant Molecular Biology , Osmania University , Hyderabad 500 007 , India., Khareedu VR; a Centre for Plant Molecular Biology , Osmania University , Hyderabad 500 007 , India., Reddy Vudem D; a Centre for Plant Molecular Biology , Osmania University , Hyderabad 500 007 , India. |
| Abstrakt: |
Andrographis nallamalayana is being widely used as tribal medicine in the treatment of leucoderma and mouth ulcers. Chemical profiling of methanolic extract of the whole plant (PE), using GC-MS and LC-MS, revealed the presence of compounds viz. α-tocopherol, β-sitosterol, tetradecanoic acid, monostearin, flavones/flavanones and their glycosides, chromones, etc. Topical application of imiquimod on the dorsal portion of male BALB/C mice resulted in the development of psoriatic symptoms (erythema, scaling, thickening and folding) with a mean disease activity index (DAI) of >7.0. Topical treatment with 100-μL PE (~6.4%/12.8%) formulations, for 12-days, resulted in the alleviation of disease symptoms. Compared to water-based formulations, emu oil-based formulation, PE400EO was found more effective in reducing the mean DAI (>84%), keratinocyte count (>65%) (p < 0.01) and interleukin-22 (~70%) (p < 0.05). We report, for the first time, anti-psoriatic activity of A. nallamalayana having great potential in developing a potent phytomedicine against psoriasis. |
