| Autor: |
Bielanski A; Agriculture Canada, Animal Diseases Research Institute, Nepean, Ontario, Canada., Eaglesome MD, Ruhnke HL, Hare WC |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of in vitro fertilization and embryo transfer : IVF [J In Vitro Fert Embryo Transf] 1989 Aug; Vol. 6 (4), pp. 236-41. |
| DOI: |
10.1007/BF01132872 |
| Abstrakt: |
Incubation of day 7 bovine embryos with 10(4) or 10(6) CFU/ml of Mycoplasma bovis (M. bovis) or microinjection of M. bovis into the cells of day 7 embryos did not influence embryonic development. M. bovis was recovered from all embryos washed 10 times by a standard pipetting method or vortexed and pipeted 10 times. M. bovis was also recovered from zonae pellucidae removed and washed from microinjected embryos. Neither treatment with trypsin nor exposure of embryos to combinations of penicillin, streptomycin, lincomycin and spectinomycin, or gentamicin, tylosin, lincomycin, and spectinomycin, inactivated M. bovis. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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