Susceptibility of northern corn rootworm Diabrotica barberi (Coleoptera: Chrysomelidae) to mCry3A and eCry3.1Ab Bacillus thuringiensis proteins.

Autor: Oyediran IO; Syngenta Crop Protection, LLC,  Research Triangle Park, NC, 27709, USA., Matthews P; Syngenta Crop Protection, LLC,  Research Triangle Park, NC, 27709, USA., Palekar N; Syngenta Crop Protection, LLC,  Research Triangle Park, NC, 27709, USA., French W; USDA ARS, North Central Agricultural Research Laboratory, Brookings, SD, 57006, USA., Conville J; Syngenta Crop Protection, LLC,  Research Triangle Park, NC, 27709, USA., Burd T; Syngenta Crop Protection, LLC,  Research Triangle Park, NC, 27709, USA.
Jazyk: angličtina
Zdroj: Insect science [Insect Sci] 2016 Dec; Vol. 23 (6), pp. 913-917. Date of Electronic Publication: 2015 Sep 24.
DOI: 10.1111/1744-7917.12249
Abstrakt: The susceptibility of the northern corn rootworm Diabrotica barberi (Smith & Lawrence) to mCry3A and eCry3.1Ab proteins derived from Bacillus thuringiensis (Bt) was determined using a diet bioassay. Northern corn rootworm neonates were exposed to different concentrations of mCry3A and eCry3.1Ab, incorporated into artificial diet. Larval mortality was evaluated after 7 d. The mCry3A and eCry3.1Ab proteins were found to be toxic to the northern corn rootworm larvae. The LC 50 and LC 99 values for mCry3A were 5.13 and 2482.31 μg/mL, respectively. For eCry3.1Ab, the LC 50 and LC 99 values were 0.49 and 213.01 μg/mL. Based on the estimated lethal concentrations, eCry3.1Ab protein was more efficacious to northern corn rootworm larvae than mCry3A. These lethal concentration values will be used as diagnostic doses for routine annual monitoring for change in susceptibility of field collected northern corn rootworm to mCry3A, and eCry3.1Ab toxins.
(© 2015 Institute of Zoology, Chinese Academy of Sciences.)
Databáze: MEDLINE
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