Generation and Characterization of Patient-Specific iPSC Model for Cardiovascular Disease.

Autor:	Lee YK; Cardiology Division, Department of Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China., Ran X; Cardiology Division, Department of Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China., Lai KW; Cardiology Division, Department of Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China., Lau VY; Cardiology Division, Department of Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China., Siu DC; Cardiology Division, Department of Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China.; Research Center of Heart, Brain, Hormone and Healthy Aging, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, China.; Shenzhen Institutes of Research and Innovation, University of Hong Kong, Hong Kong, China.; Division of Cardiology, Queen Mary Hospital, Hong Kong, China., Tse HF; Cardiology Division, Department of Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China. hftse@hku.hk.; Research Center of Heart, Brain, Hormone and Healthy Aging, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, China. hftse@hku.hk.; Shenzhen Institutes of Research and Innovation, University of Hong Kong, Hong Kong, China. hftse@hku.hk.; Hong Kong-Guangdong Joint Laboratory on Stem Cell and Regenerative Medicine, Hong Kong, China. hftse@hku.hk.; Division of Cardiology, Queen Mary Hospital, Hong Kong, China. hftse@hku.hk.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2016; Vol. 1353, pp. 191-213.
DOI:	10.1007/7651_2015_273
Abstrakt:	Advances in differentiation of cardiomyocytes from human induced pluripotent stem cell (hiPSC) were emerged as a tool for modeling of cardiovascular disease that recapitulates the phenotype for the purpose of drug screening, biomarker discovery, and testing of single-nucleotide polymorphism (SNP) as a modifier for disease stratification. Here, we describe the (1) retroviral reprogramming strategies in the generation of human iPSC, (2) methodology in characterization of iPSC in order to identify the stem cell clones with the best quality, and (3) protocol of cardiac differentiation by modulation of Wnt signaling and β-catenin pathway.
Databáze:	MEDLINE
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