Analytical Validation of AmpliChip p53 Research Test for Archival Human Ovarian FFPE Sections.
| Autor: | Marton MJ; Merck Research Laboratories, Molecular Biomarkers and Diagnostics, Rahway, New Jersey, United States of America., McNamara AR; Roche Molecular Systems, Inc., Pleasanton, California, United States of America., Nikoloff DM; Roche Molecular Systems, Inc., Pleasanton, California, United States of America., Nakao A; Roche Molecular Systems, Inc., Pleasanton, California, United States of America., Cheng J; Merck Research Laboratories, Clinical Oncology, North Wales, Pennsylvania, United States of America. |
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| Jazyk: | angličtina |
| Zdroj: | PloS one [PLoS One] 2015 Jun 30; Vol. 10 (6), pp. e0131497. Date of Electronic Publication: 2015 Jun 30 (Print Publication: 2015). |
| DOI: | 10.1371/journal.pone.0131497 |
| Abstrakt: | The p53 tumor suppressor gene (TP53) is reported to be mutated in nearly half of all tumors and plays a central role in genome integrity. Detection of mutations in p53 can be accomplished by many assays, including the AmpliChip p53 Research Test. The AmpliChip p53 Research Test has been successfully used to determine p53 status in hematologic malignancies and fresh frozen solid tissues but there are few reports of using the assay with formalin fixed, paraffin-embedded (FFPE) tissue. The objective of this study was to describe analytical performance characterization of the AmpliChip p53 Research Test to detect p53 mutations in genomic DNA isolated from archival FFPE human ovarian tumor tissues. Method correlation with sequencing showed 96% mutation-wise agreement and 99% chip-wise agreement. We furthermore observed 100% agreement (113/113) of the most prevalent TP53 mutations. Workflow reproducibility was 96.8% across 8 samples, with 2 operators, 2 reagent lots and 2 instruments. Section-to-section reproducibility was 100% for each sample across a 60 μm region of the FFPE block from ovarian tumors. These data indicate that the AmpliChip p53 Research Test is an accurate and reproducible method for detecting mutations in TP53 from archival FFPE human ovarian specimens. |
| Databáze: | MEDLINE |
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