New glycosylated conjugate copolymer N-acetyl-β-D-glucosaminyl-pluronic: Synthesis, self-assembly and biological assays.

Autor: Frizon TE; Laboratório de Processamento de Polímeros Avançados - LAPPA, Universidade do Extremo Sul Catarinense, 88806-000 Criciúma, SC, Brazil., Micheletto YM; Laboratório de Processamento de Polímeros Avançados - LAPPA, Universidade do Extremo Sul Catarinense, 88806-000 Criciúma, SC, Brazil., Westrup JL; Laboratório de Processamento de Polímeros Avançados - LAPPA, Universidade do Extremo Sul Catarinense, 88806-000 Criciúma, SC, Brazil., Wakabayashi PS; Laboratório de Processamento de Polímeros Avançados - LAPPA, Universidade do Extremo Sul Catarinense, 88806-000 Criciúma, SC, Brazil., Serafim FR; Laboratório de Processamento de Polímeros Avançados - LAPPA, Universidade do Extremo Sul Catarinense, 88806-000 Criciúma, SC, Brazil., Damiani AP; Laboratório de Biologia Celular e Molecular, PPGCS, Universidade do Extremo Sul Catarinense, Av. Universitária, 1105, 88806-000 Criciúma, SC, Brazil., Longaretti LM; Laboratório de Biologia Celular e Molecular, PPGCS, Universidade do Extremo Sul Catarinense, Av. Universitária, 1105, 88806-000 Criciúma, SC, Brazil., de Andrade VM; Laboratório de Biologia Celular e Molecular, PPGCS, Universidade do Extremo Sul Catarinense, Av. Universitária, 1105, 88806-000 Criciúma, SC, Brazil., Giacomelli FC; Centro de Ciências Naturais e Humanas, Universidade Federal do ABC, 09210-170 Santo André, SP, Brazil., Fort S; Univ Grenoble Alpes, CERMAV, F-38000 Grenoble, France., Dal Bó AG; Laboratório de Processamento de Polímeros Avançados - LAPPA, Universidade do Extremo Sul Catarinense, 88806-000 Criciúma, SC, Brazil; Univ Grenoble Alpes, CERMAV, F-38000 Grenoble, France. Electronic address: adalbo@unesc.net.
Jazyk: angličtina
Zdroj: Colloids and surfaces. B, Biointerfaces [Colloids Surf B Biointerfaces] 2015 Sep 01; Vol. 133, pp. 323-30. Date of Electronic Publication: 2015 Jun 22.
DOI: 10.1016/j.colsurfb.2015.06.035
Abstrakt: This work describes the synthesis of a new glycosylated conjugate copolymer, GlcNAc-PEO75-PPO30-PEO75-GlcNAc (GlcNAc-PluronicF68-GlcNAc), using click chemistry from Pluronic(®) F68 and propargyl-2-N-acetamido-2-deoxy-β-D-glucopyranoside. Micelles were prepared by the self-assembly of GlcNAc-PluronicF68-GlcNAc in phosphate-buffered solution. The critical micelle concentration was determined by fluorescence spectroscopy, and the value was found to be equal to 5.8mgmL(-1). The Gibbs free energy (ΔG) of micellization is negative, indicating that the organization of amphiphiles is governed by the hydrophobic effects in an entropy-driven process. The scattering characterization of GlcNAc-PluronicF68-GlcNAc micelles showed a hydrodynamic radius of 8.7nm and negative zeta potential (-21.0±0.9mV). The TEM image evidences the spherical shape of the objects self-assemble into highly regular micelles having a mean diameter of 10nm. The SAXS profile confirmed the spherical shape of the assemblies comprising a swollen PPO core (Rcore=2.25nm) stabilized by PEO chains following Gaussian statistics. The results of the comet assay showed that the GlcNAc-PluronicF68-GlcNAc micelles were not genotoxic, and the cell viability test was higher than 97% for all concentrations, demonstrating that GlcNAc-PluronicF68-GlcNAc is not toxic.
(Copyright © 2015 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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