Development of a High-Throughput Functional Screen Using Nanowell-Assisted Cell Patterning.

Autor:	Ozkumur AY; Electrical and Electronics Engineering Department, Bahcesehir University, Istanbul, 34349, Turkey., Goods BA; Department of Biological Engineering, Koch Institute for Integrative Cancer Research at MIT, Cambridge, MA, 02139, USA., Love JC; Department of Chemical Engineering, Koch Institute for Integrative Cancer Research at MIT, Cambridge, MA, 02139, USA.; The Broad Institute of MIT and Harvard, Cambridge, MA, 02142, USA.; The Ragon Institute of MGH, MIT and Harvard, Cambridge, MA, 02139, USA.
Jazyk:	angličtina
Zdroj:	Small (Weinheim an der Bergstrasse, Germany) [Small] 2015 Sep; Vol. 11 (36), pp. 4643-50. Date of Electronic Publication: 2015 Jun 29.
DOI:	10.1002/smll.201500674
Abstrakt:	Living-cell-based screens can facilitate lead discovery of functional therapeutics of interest. A versatile and scalable method is reported that uses dense arrays of nanowells for imparting defined patterns on monolayers of cells. It is shown that this approach can coordinate a multi-component biological assay by designing and implementing a high-throughput, functional nanoliter-scale neutralization assay to identify neutralizing antibodies against HIV. (© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
Databáze:	MEDLINE
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